GAP43 Polyclonal antibody

GAP43 Polyclonal Antibody for IF, IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (4)

Applications

WB, IP, IHC, IF, CoIP, ELISA

Conjugate

Unconjugated

Publications

47

Reviews (1)

Cat no : 16971-1-AP

Synonyms

Axonal membrane protein GAP 43, B 50, GAP43, growth associated protein 43, Neural phosphoprotein B 50, Neuromodulin, PP46

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Validation Data Gallery

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  WB analysis using 16971-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 16971-1-AP (GAP43 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse brain using 16971-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 16971-1-AP (GAP43 antibody) at dilution of 1:1200 incubated at room temperature for 1.5 hours.

• 

  WB analysis of rat lung using 16971-1-AP

  rat lung tissue were subjected to SDS PAGE followed by western blot with 16971-1-AP (GAP43 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  IP experiment of mouse brain using 16971-1-AP

  IP result of anti-GAP43 (IP:16971-1-AP, 4ug; Detection:16971-1-AP 1:500) with mouse brain tissue lysate 6000ug.

• 

  IHC staining of mouse brain using 16971-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 16971-1-AP (GAP43 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse brain using 16971-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 16971-1-AP (GAP43 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of SH-SY5Y using 16971-1-AP

  Immunofluorescent analysis of (4% PFA) fixed SH-SY5Y cells using GAP43 antibody (16971-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red).

• 

  IF Staining of mouse brain using 16971-1-AP

  Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using GAP43 antibody (16971-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Tested Applications

• Positive WB detected in: mouse brain tissue, rat lung tissue, rat brain tissue
• Positive IP detected in: mouse brain tissue
• Positive IHC detected in: mouse brain tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF detected in: mouse brain tissue, SH-SY5Y cells

Recommended dilution

• Western Blot (WB): WB : 1:2000-1:10000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF): IF : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 32 publications below
• IHC: See 8 publications below
• IF: See 13 publications below
• IP: See 1 publications below
• CoIP: See 1 publications below

Product Information

16971-1-AP targets GAP43 in WB, IP, IHC, IF, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse, rat, Gekko japonicus, zebrafish, hamster, canine
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: GAP43 fusion protein Ag9294
• Full Name: growth associated protein 43
• Calculated Molecular Weight: 238 aa, 25 kDa
• Observed Molecular Weight: 43 kDa
• GenBank Accession Number: BC007936
• Gene Symbol: GAP43
• Gene ID (NCBI): 2596
• RRID: AB_2278881
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

The neuronal growth-associated protein GAP43 is also known as neuromodulin, B-50, P-57, F1 and pp46. Deficiency of GAP43 in mice results in death early in the postnatal period. GAP43 is one of the main substrates for protein kinase C in the brain. GAP43 is an intracellular growth-associated protein that appears to assist neuronal pathfinding and branching during development and regeneration, and may contribute to presynaptic membrane changes in the adult, leading to the neurotransmitter release, endocytosis and synaptic vesicle recycling, long-term potentiation, spatial memory formation, and learning. The predicated molecular weight of about 25 kDa is much lower than the apparent observed molecular weight of 43 kDa on SDS-PAGE gels, and this occurs because the highly charged nature of GAP43 causes it to bind less than the average amount of SDS per amino acid, and because the protein has an elongated structure.

Publications

Species	Application	Title
rat	WB	Adv Sci (Weinh); An Important Function of Petrosiol E in Inducing the Differentiation of Neuronal Progenitors and in Protecting Them against Oxidative Stress.; Authors - Jing Liu
rat	WB	Mater Today Bio; Ropivacaine microsphere-loaded electroconductive nerve dressings for long-acting analgesia and functional recovery following diabetic peripheral nerve injury; Authors - Fangguo Liang
rat	WB	Phytomedicine; Ginsenoside Rb1 protects hippocampal neurons in depressed rats based on mitophagy-regulated astrocytic pyroptosis; Authors - Yannan Li
rat	WB	Environ Pollut; Combined exposure of lead and high-fat diet enhanced cognitive decline via interacting with CREB-BDNF signaling in male rats.; Authors - Rundong Liu
rat	WB	Aging (Albany NY); Enriched environment remedies cognitive dysfunctions and synaptic plasticity through NMDAR-Ca2+-Activin A circuit in chronic cerebral hypoperfusion rats.; Authors - Xin Zhang
mouse	WB	Sci Total Environ; Neurotoxicity and biomarkers of zinc oxide nanoparticles in main functional brain regions and dopaminergic neurons.; Authors - Huanliang Liu

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Azita (Verified Customer) (05-31-2021)

Human primary cortical neurons was labelled with 1/500 GAP43. Very strong labelling.

• Applications: Immunohistochemistry
• Primary Antibody Dilution: 1/500
• Cell Tissue Type: Human primary cortical neurons

GAP43 Antibody Immunohistochemistry validation (1/500 dilution) in Human primary cortical neurons (Cat no:16971-1-AP)

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