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  • KD/KO Validated

GANP Polyclonal antibody

GANP Polyclonal Antibody for WB, IHC, IF/ICC, FC (Intra), IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (1)

Applications

WB, IHC, IF/ICC, FC (Intra), IP, ELISA

Conjugate

Unconjugated

Cat no : 11054-1-AP

Synonyms

MCM3AP, MAP80, KIAA0572, Germinal-center associated nuclear protein, EC:2.3.1.48



Tested Applications

Positive WB detected inmouse brain tissue, NIH/3T3 cells
Positive IP detected inmouse brain tissue
Positive IHC detected inhuman colon cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHepG2 cells
Positive FC (Intra) detected inHepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:200-1:1000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:10-1:100
Immunofluorescence (IF)/ICCIF/ICC : 1:20-1:200
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

11054-1-AP targets GANP in WB, IHC, IF/ICC, FC (Intra), IP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, monkey
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen GANP fusion protein Ag1486
Full Name minichromosome maintenance complex component 3 associated protein
Calculated Molecular Weight 218 kDa
Observed Molecular Weight 75-80 kDa
GenBank Accession NumberBC013285
Gene Symbol GANP
Gene ID (NCBI) 8888
RRIDAB_2266280
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

The minichromosome maintenance protein 3 (MCM3) is one of the MCM proteins essential for the initiation of DNA replication. MCM3AP is MCM3 associated protein, and it has phosphorylation-dependent DNA-primase activity, which was up-regulated in antigen immunization induced germinal center. The mutagenesis of a nuclear localization signal of MCM3 affects the binding of this protein with MCM3, suggesting that MCM3AP may also facilitate MCM3 nuclear localization. Besides, MCM3AP is an acetyltransferase cotaining putative acetyl CoA binding motifs conserved within the GCN5-related N-acetyltransferase superfamily.

Protocols

Product Specific Protocols
WB protocol for GANP antibody 11054-1-APDownload protocol
IHC protocol for GANP antibody 11054-1-APDownload protocol
IF protocol for GANP antibody 11054-1-APDownload protocol
IP protocol for GANP antibody 11054-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Nat Commun

GANP regulates recruitment of AID to immunoglobulin variable regions by modulating transcription and nucleosome occupancy.

Authors - Singh Shailendra Kumar SK
  • KD Validated
humanWB

J Immunol

GANP Interacts with APOBEC3G and Facilitates Its Encapsidation into the Virions to Reduce HIV-1 Infectivity.

Authors - Maeda Kazuhiko K
  • KD Validated
humanWB

Front Mol Neurosci

The Effect of MCM3AP-AS1/miR-211/KLF5/AGGF1 Axis Regulating Glioblastoma Angiogenesis.

Authors - Chunqing Yang
  • KD Validated
human,monkeyWB,IF

J Biol Chem

GANP-mediated recruitment of activation-induced cytidine deaminase to cell nuclei and to immunoglobulin variable region DNA.

Authors - Maeda Kazuhiko K