Various lysates were subjected to SDS PAGE followed by western blot with 83324-1-RR (GANAB antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.
Various lysates were subjected to SDS PAGE followed by western blot with 83324-1-RR (GANAB antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.
WB analysis of HeLa using 83324-1-RR
WB result of GANAB antibody (83324-1-RR; 1:3000; incubated at room temperature for 1.5 hours) with sh-Control and sh-GANAB transfected HeLa cells.
Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 83324-1-RR (GANAB antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IF Staining of HeLa using 83324-1-RR
Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using GANAB antibody (83324-1-RR, Clone: 240279B7 ) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2).
Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using GANAB antibody (83324-1-RR, Clone: 240279B7 ) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2).
FC experiment of HeLa using 83324-1-RR
1x10^6 HeLa cells were intracellularly stained with 0.25 ug GANAB Recombinant antibody (83324-1-RR, Clone:240279B7)(red) or Isotype Controland (blue) and APC-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).
1x10^6 HeLa cells were intracellularly stained with 0.25 ug GANAB Recombinant antibody (83324-1-RR, Clone:240279B7)(red) or Isotype Controland (blue) and APC-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).
Affinity and Kinetic Characterization of 83324-1-RR
Biolayer interferometry (BLl) kinetic assays of 83324-1-RR against Human GANAB were performed. The affinity constant is 0.186 nM.
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HeLa cells, A431 cells, HepG2 cells, mouse kidney tissue, mouse liver tissue, rat liver tissue, human placenta tissue
Positive IHC detected in
mouse liver tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected in
HeLa cells
Positive FC (Intra) detected in
HeLa cells
Recommended dilution
Application
Dilution
Western Blot (WB)
WB : 1:5000-1:50000
Immunohistochemistry (IHC)
IHC : 1:50-1:500
Immunofluorescence (IF)/ICC
IF/ICC : 1:50-1:500
Flow Cytometry (FC) (INTRA)
FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.
Product Information
83324-1-RR targets GANAB in WB, IHC, IF/ICC, FC (Intra), ELISA applications and shows reactivity with human, mouse, rat samples.
Various lysates were subjected to SDS PAGE followed by western blot with 83324-1-RR (GANAB antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.
WB analysis of HeLa using 83324-1-RR
WB result of GANAB antibody (83324-1-RR; 1:3000; incubated at room temperature for 1.5 hours) with sh-Control and sh-GANAB transfected HeLa cells.
IHC Figures
IHC staining of mouse liver using 83324-1-RR
Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 83324-1-RR (GANAB antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IF/ICC Figures
IF Staining of HeLa using 83324-1-RR
Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using GANAB antibody (83324-1-RR, Clone: 240279B7 ) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2).
FC (INTRA) Figures
FC experiment of HeLa using 83324-1-RR
1x10^6 HeLa cells were intracellularly stained with 0.25 ug GANAB Recombinant antibody (83324-1-RR, Clone:240279B7)(red) or Isotype Controland (blue) and APC-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).
AFFINITY Figures
Affinity and Kinetic Characterization of 83324-1-RR
Biolayer interferometry (BLl) kinetic assays of 83324-1-RR against Human GANAB were performed. The affinity constant is 0.186 nM.
The species listed in Tested Reactivity are in-house verified and applicable species. For unlisted species, please refer to the homology analysis of the immunogen sequence and related species. For rabbit polyclonal antibodies, homology >70% is recommended. For mouse monoclonal antibodies and rabbit recombinant antibodies, homology >90% is recommended. Generally, the higher the homology, the greater the applicability. However, there will be certain differences in protein expression in different species, tissues or cells. Therefore, the homology analysis results are for reference only and do not serve as a guarantee.
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Proteintech
KD/KO VALIDATED
GANAB Recombinant antibody
Catalog Number
83324-1-RR
Citations
-
Dilutions
WB : 1:5000-1:50000 IHC : 1:50-1:500 IF/ICC : 1:50-1:500 FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension
Applications
WB, IHC, IF/ICC, FC (Intra), ELISA
Reactivity
human, mouse, rat
Product Guarantee
Covers any species including not listed on datasheet
Covers any applications including not listed on datasheet
