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GALM Polyclonal antibody

GALM Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 16022-1-AP

Synonyms

Aldose 1 epimerase, BLOCK25, Galactose mutarotase, GALM, IBD1



Tested Applications

Positive WB detected inHeLa cells, HepG2 cells
Positive IHC detected inhuman heart tissue, human brain tissue, human spleen tissue, mouse spleen tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:8000
Immunohistochemistry (IHC)IHC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

16022-1-AP targets GALM in WB, IHC, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Cited Reactivityhuman
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen GALM fusion protein Ag8842
Full Name galactose mutarotase (aldose 1-epimerase)
Calculated Molecular Weight 342 aa, 38 kDa
Observed Molecular Weight 38 kDa
GenBank Accession NumberBC019263
Gene Symbol GALM
Gene ID (NCBI) 130589
RRIDAB_2108708
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

GALM, also named as Aldose 1-epimerase, belongs to the aldose epimerase family. GALM is a mutarotase that catalyzes the interconversion of beta-D-galactose and alpha-D-galactose during galactose metabolism. GALM is involved in the maintenance of the equilibrium between the beta- and alpha- anomers of galactose, therefore ensuring a sufficient supply of the alpha-anomer for GALK1. Galm is also active on D-glucose, but its preference for galactose is higher than that of glucose. The calculated molecular weight of GALM is 38 kDa.

Protocols

Product Specific Protocols
WB protocol for GALM antibody 16022-1-APDownload protocol
IHC protocol for GALM antibody 16022-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Genet Med

Biallelic GALM pathogenic variants cause a novel type of galactosemia.

Authors - Yoichi Wada
humanWB,IHC

Front Cell Dev Biol

Comprehensive Analyses of Glucose Metabolism in Glioma Reveal the Glioma-Promoting Effect of GALM.

Authors - Jiacheng Xu
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