GALE Monoclonal antibody

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  WB analysis using 67772-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 67772-1-Ig (GALE antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

GALE Monoclonal Antibody for WB, ELISA

Cat No. 67772-1-Ig

Clone No.3F2C2

Host / Isotype

Mouse / IgG2b

Reactivity

Human, Mouse, Rat, Pig, Rabbit

Applications

WB, ELISA

Synonyms

FLJ95174, FLJ97302, Galactowaldenase, GALE, SDR1E1, UDP galactose 4 epimerase, UDP glucose 4 epimerase

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Tested Applications

• Positive WB detected in: U2OS cells, A375 cells, HEK-293T cells, HeLa cells, K-562 cells, 4T1 cells, rat liver tissue, pig liver tissue, rabbit liver tissue

Recommended dilution

• Western Blot (WB): WB : 1:5000-1:50000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

67772-1-Ig targets GALE in WB, ELISA applications and shows reactivity with Human, Mouse, Rat, Pig, Rabbit samples.

• Tested Reactivity: Human, Mouse, Rat, Pig, Rabbit
• Host / Isotype: Mouse / IgG2b
• Class: Monoclonal
• Type: Antibody
• Immunogen: GALE fusion protein Ag6571
• Full Name: UDP-galactose-4-epimerase
• Calculated Molecular Weight: 38 kDa
• Observed Molecular Weight: 38 kDa
• GenBank Accession Number: BC050685
• Gene Symbol: GALE
• Gene ID (NCBI): 2582
• RRID: AB_2918537
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: Q14376
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

GALE(Galactowaldenase) is also named as UDP-glucose 4-epimerase, UDP-galactose 4-epimerase, and belongs to the sugar epimerase family. UDP-galactose 4-epimerase catalyzes the interconversion of UDP-glucose and UDP-galactose during normal galactose metabolism. In humans, deficiencies in this enzyme lead to the complex disorder referred to as epimerase-deficiency galactosemia(PMID:10801319).It can exsit as a homodimer(PMID:8931134).