GALE Polyclonal antibody

• Featured Product
• KD/KO Validated

GALE Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse and More (1)

Applications

WB, IHC, IF/ICC, IP, ELISA

Conjugate

Unconjugated

Publications

4

Cat no : 14414-1-AP

Synonyms

UDP-GlcNAc 4-epimerase, UDP-GalNAc 4-epimerase, UDP-galactose 4-epimerase, SDR1E1, EC:5.1.3.7

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Validation Data Gallery

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  WB analysis of A375 using 14414-1-AP

  A375 cells were subjected to SDS PAGE followed by western blot with 14414-1-AP (GALE antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  WB analysis of MCF-7 using 14414-1-AP

  MCF7 cells were subjected to SDS PAGE followed by western blot with 14414-1-AP (GALE antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 14414-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 14414-1-AP (GALE antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  IP experiment of HeLa using 14414-1-AP

  IP result of anti-GALE (IP:14414-1-AP, 4ug; Detection:14414-1-AP 1:300) with HeLa cells lysate 1080ug.

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  IHC staining of human malignant melanoma using 14414-1-AP

  Immunohistochemical analysis of paraffin-embedded human malignant melanoma using 14414-1-AP (GALE antibody) at dilution of 1:100 (under 10x lens).

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  IHC staining of human malignant melanoma using 14414-1-AP

  Immunohistochemical analysis of paraffin-embedded human malignant melanoma using 14414-1-AP (GALE antibody) at dilution of 1:100 (under 40x lens).

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  IF Staining of HeLa using 14414-1-AP

  Immunofluorescent analysis of (-20℃ Ethanol) fixed HeLa cells using 14414-1-AP (GALE antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

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Tested Applications

• Positive WB detected in: A375 cells, HeLa cells, MCF-7 cells
• Positive IP detected in: HeLa cells
• Positive IHC detected in: human malignant melanoma tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: HeLa cells

Recommended dilution

• Western Blot (WB): WB : 1:200-1:1000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:20-1:200
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 1 publications below
• WB: See 4 publications below
• IHC: See 2 publications below

Product Information

14414-1-AP targets GALE in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Cited Reactivity: human, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: GALE fusion protein Ag5756
• Full Name: UDP-galactose-4-epimerase
• Calculated Molecular Weight: 38 kDa
• Observed Molecular Weight: 38 kDa
• GenBank Accession Number: BC050685
• Gene Symbol: GALE
• Gene ID (NCBI): 2582
• RRID: AB_2108540
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

GALE(Galactowaldenase) is also named as UDP-glucose 4-epimerase, UDP-galactose 4-epimerase, and belongs to the sugar epimerase family. UDP-galactose 4-epimerase catalyzes the interconversion of UDP-glucose and UDP-galactose during normal galactose metabolism. In humans, deficiencies in this enzyme lead to the complex disorder referred to as epimerase-deficiency galactosemia(PMID:10801319).It can exsit as a homodimer(PMID:8931134).

Publications

Species	Application	Title
rat	WB	Redox Biol; Hypoxic acclimation improves cardiac redox homeostasis and protects heart against ischemia-reperfusion injury through upregulation of O-GlcNAcylation.; Authors - Wei Ou
human	WB, IHC	PLoS One; Angelica Sinensis Polysaccharides Stimulated UDP-Sugar Synthase Genes through Promoting Gene Expression of IGF-1 and IGF1R in Chondrocytes: Promoting Anti-Osteoarthritic Activity.; Authors - Yinxian Wen
human	WB	Infect Drug Resist; iTRAQ-Based Proteomics Reveals Potential Anti-Virulence Targets for ESBL-Producing Klebsiella pneumoniae.; Authors - Yan Wang
human	WB, IHC	Biochem Biophys Res Commun; The critical role of UDP-galactose-4-epimerase in osteoarthritis: modulating proteoglycans synthesis of articular chondrocytes.; Authors - Yinxian Wen ; KD Validated