GAGE2D Polyclonal antibody, PBS Only

GAGE2D Polyclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, IF/ICC, Indirect ELISA

Conjugate

Unconjugated

Cat no : 12532-1-PBS

Synonyms

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Validation Data Gallery

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  WB analysis of K-562 using 12532-1-AP (same clone as 12532-1-PBS)

  K-562 cells were subjected to SDS PAGE followed by western blot with 12532-1-AP (GAGE2D antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12532-1-PBS in a different storage buffer formulation.

• 

  WB analysis of human testis using 12532-1-AP (same clone as 12532-1-PBS)

  human testis tissue were subjected to SDS PAGE followed by western blot with 12532-1-AP (GAGE2D antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12532-1-PBS in a different storage buffer formulation.

• 

  WB analysis of HeLa using 12532-1-AP (same clone as 12532-1-PBS)

  HeLa cells were subjected to SDS PAGE followed by western blot with 12532-1-AP (GAGE2D antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12532-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human liver cancer using 12532-1-AP (same clone as 12532-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 12532-1-AP (GAGE2D Antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 12532-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human liver cancer using 12532-1-AP (same clone as 12532-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 12532-1-AP (GAGE2D Antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 12532-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human testis using 12532-1-AP (same clone as 12532-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 12532-1-AP (GAGE2D Antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 12532-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human testis using 12532-1-AP (same clone as 12532-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 12532-1-AP (GAGE2D Antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 12532-1-PBS in a different storage buffer formulation.

• 

  IF Staining of HepG2 using 12532-1-AP (same clone as 12532-1-PBS)

  Immunofluorescent analysis of HepG2 cells, using GAGE2D antibody 12532-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red). This data was developed using the same antibody clone with 12532-1-PBS in a different storage buffer formulation.

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Product Information

12532-1-PBS targets GAGE2D in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: GAGE2D fusion protein Ag3230
• Full Name: G antigen 2D
• Calculated Molecular Weight: 116 aa, 13 kDa
• Observed Molecular Weight: 18-22 kDa
• GenBank Accession Number: BC018052
• Gene Symbol: GAGE2D
• Gene ID (NCBI): 729408
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.

Background Information

GAGE2D, also named as GAGE8 and CT4.8, is expressed in testis and tumor tissues.