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GADD45GIP1 Recombinant antibody, PBS Only

GADD45GIP1 Uni-rAbTM Recombinant Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, ELISA

Conjugate

Unconjugated

CloneNo.

240947D1

Cat no : 83823-5-PBS

Synonyms

CRIF1, CR6-interacting factor 1, CKII beta-associating protein, CKBBP2, 39S ribosomal protein L59, mitochondrial

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using GADD45GIP1 Recombinant antibody (83823-5-RR)

    WB analysis using 83823-5-RR (same clone as 83823-5-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 83823-5-RR (GADD45GIP1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 83823-5-PBS in a different storage buffer formulation.

Product Information

The immunogen of 83823-5-PBS is GADD45GIP1 Fusion Protein expressed in E. coli.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen GADD45GIP1 fusion protein Ag9299
Full Name growth arrest and DNA-damage-inducible, gamma interacting protein 1
Calculated Molecular Weight 222 aa, 25 kDa
Observed Molecular Weight 25-28 kDa
GenBank Accession NumberBC013027
Gene Symbol GADD45GIP1
Gene ID (NCBI) 90480
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

Growth arrest and DNA-damage-inducible proteins-interacting protein 1(GADD45GIP1), also known as CRIF1, is newly identified de novo components in large subunit of mitoribosome. It is essential for the translation of mitochondrial oxidative phosphorylation (OXPHOS) polypeptides in mammalian mitochondria. The essential role of CRIF1 in mitochondrial synthesis and membrane integration of OXPHOS polypeptides was shown in brain-specific CRIF1-deficient mice, which exhibited profound OXPHOS failure and marked neurodegeneration. (PMID 22819524) In addition, GADD45GIP1 enhances the function of GADD45 in maintaining genome stability, DNA repair and cell cycle regulation by interacting with the GADD45 protein family. It's reported that GADD45GIP1 may be induced by p53, a process that is closely related to the cell's response to DNA damage.(PMID:28599472)