GADD45GIP1 Recombinant antibody, PBS Only

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  WB analysis using 83823-5-RR (same clone as 83823-5-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 83823-5-RR (GADD45GIP1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 83823-5-PBS in a different storage buffer formulation.

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  IHC staining of human ovarian cancer using 83823-5-RR (same clone as 83823-5-PBS)

  Immunohistochemical analysis of paraffin-embedded human ovarian cancer slide using 83823-5-RR (GADD45GIP1 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 83823-5-PBS in a different storage buffer formulation.

• 

  IHC staining of human ovarian cancer using 83823-5-RR (same clone as 83823-5-PBS)

  Immunohistochemical analysis of paraffin-embedded human ovarian cancer slide using 83823-5-RR (GADD45GIP1 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 83823-5-PBS in a different storage buffer formulation.

GADD45GIP1 {ptg:Brand} Recombinant Antibody for WB, IHC, ELISA

Cat No. 83823-5-PBS

Clone No.240947D1

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, ELISA

Synonyms

CRIF1, CR6-interacting factor 1, CKII beta-associating protein, CKBBP2, 39S ribosomal protein L59, mitochondrial

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Product Information

83823-5-PBS targets GADD45GIP1 in WB, IHC, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: GADD45GIP1 fusion protein Ag9299
• Full Name: growth arrest and DNA-damage-inducible, gamma interacting protein 1
• Calculated Molecular Weight: 222 aa, 25 kDa
• Observed Molecular Weight: 25-28 kDa
• GenBank Accession Number: BC013027
• Gene Symbol: GADD45GIP1
• Gene ID (NCBI): 90480
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purfication
• UNIPROT ID: Q8TAE8
• Storage Buffer: PBS only , pH 7.3
• Storage Conditions: Store at -80°C.

Background Information

Growth arrest and DNA-damage-inducible proteins-interacting protein 1(GADD45GIP1), also known as CRIF1, is newly identified de novo components in large subunit of mitoribosome. It is essential for the translation of mitochondrial oxidative phosphorylation (OXPHOS) polypeptides in mammalian mitochondria. The essential role of CRIF1 in mitochondrial synthesis and membrane integration of OXPHOS polypeptides was shown in brain-specific CRIF1-deficient mice, which exhibited profound OXPHOS failure and marked neurodegeneration. (PMID 22819524) In addition, GADD45GIP1 enhances the function of GADD45 in maintaining genome stability, DNA repair and cell cycle regulation by interacting with the GADD45 protein family. It's reported that GADD45GIP1 may be induced by p53, a process that is closely related to the cell's response to DNA damage.(PMID:28599472)