GADD45A Polyclonal antibody

• Featured Product
• KD/KO Validated

GADD45A Polyclonal Antibody for ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Publications

12

Cat no : 13747-1-AP

Synonyms

DDIT 1, DDIT1, GADD45, GADD45A

---

Validation Data Gallery

• 

  WB analysis of mouse spleen using 13747-1-AP

  mouse spleen tissue were subjected to SDS PAGE followed by western blot with 13747-1-AP (GADD45A Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse lung using 13747-1-AP

  mouse lung tissue were subjected to SDS PAGE followed by western blot with 13747-1-AP (GADD45A Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of K-562 using 13747-1-AP

  K-562 cells were subjected to SDS PAGE followed by western blot with 13747-1-AP (GADD45A Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of K-562 using 13747-1-AP

  K-562 cells were subjected to SDS PAGE followed by western blot with 13747-1-AP (GADD45A Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of COLO 320 using 13747-1-AP

  COLO 320 cells were subjected to SDS PAGE followed by western blot with 13747-1-AP (GADD45A Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of PC-13 using 13747-1-AP

  PC-13 cells were subjected to SDS PAGE followed by western blot with 13747-1-AP (GADD45A Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of A549 using 13747-1-AP

  A549 cells were subjected to SDS PAGE followed by western blot with 13747-1-AP (GADD45A Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

---

Tested Applications

Recommended dilution

Application	Dilution
Sample-dependent, check data in validation data gallery

Published Applications

• KD/KO: See 1 publications below
• WB: See 12 publications below
• IHC: See 1 publications below

Product Information

13747-1-AP targets GADD45A in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: GADD45A fusion protein Ag4687
• Full Name: growth arrest and DNA-damage-inducible, alpha
• Calculated molecular weight: 165 aa, 18 kDa
• GenBank accession number: BC011757
• Gene symbol: GADD45A
• Gene ID (NCBI): 1647
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

GADD45A, also named as DDIT1 and GADD45, belongs to the GADD45 family. It binds to proliferating cell nuclear antigen. GADD45A may affect PCNA interaction with some CDK (cell division protein kinase) complexes; stimulates DNA excision repair in vitro and inhibits entry of cells into S phase. GADD45A plays an essential role in active DNA demethylation during terminal osteogenic differentiation of adipose-derived mesenchymal stem cells. GADD45A has Dimer (~28-36 kDa) and Monomer (14-18 kDa) forms (PMID:11498536).

Publications

Species	Application	Title
human	WB	Biochem Pharmacol; Selective activation of tumor-suppressive MAPKP signaling pathway by triptonide effectively inhibits pancreatic cancer cell tumorigenicity and tumor growth.; Authors - Bin Zhang
mouse	WB	Bosn J Basic Med Sci; CircRNA_014511 affects the radiosensitivity of bone marrow mesenchymal stem cells by binding to miR-29b-2-5p.; Authors - Yanjie Wang
rat	WB	Int J Nanomedicine; TiO2 Nanoparticles Caused DNA Damage in Lung and Extra-Pulmonary Organs Through ROS-Activated FOXO3a Signaling Pathway After Intratracheal Administration in Rats.; Authors - Bin Han
rat	WB	Neural Regen Res; Silencing Huwe1 reduces apoptosis of cortical neurons exposed to oxygen-glucose deprivation and reperfusion.; Authors - Guo-Qian He
human	WB	Tumour Biol; Overexpression of HP1γ is associated with poor prognosis in non-small cell lung cancer cell through promoting cell survival.; Authors - Ji Zhou
human	WB	Onco Targets Ther; GADD45a Mediated Cell Cycle Inhibition Is Regulated By P53 In Bladder Cancer.; Authors - Na Han