GADD45A Polyclonal antibody

• 

  WB analysis using 13747-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 13747-1-AP (GADD45A antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• Featured Product
• KD/KO Validated

GADD45A Polyclonal Antibody for WB, ELISA

Cat No. 13747-1-AP

Publications(18)

Reviews (1)

Host / Isotype

Rabbit / IgG

Reactivity

human and More (2)

Applications

WB, IHC, ELISA

Synonyms

DDIT 1, DDIT1, GADD45, GADD45A

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Tested Applications

• Positive WB detected in: HEK-293 cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 1 publications below
• WB: See 18 publications below
• IHC: See 1 publications below

Product Information

13747-1-AP targets GADD45A in WB, IHC, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Cited Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: GADD45A fusion protein Ag4687
• Full Name: growth arrest and DNA-damage-inducible, alpha
• Calculated Molecular Weight: 165 aa, 18 kDa
• Observed Molecular Weight: 18 kDa, 33 kDa
• GenBank Accession Number: BC011757
• Gene Symbol: GADD45A
• Gene ID (NCBI): 1647
• RRID: AB_2108058
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: P24522
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

GADD45A, also named as DDIT1 and GADD45, belongs to the GADD45 family. It binds to proliferating cell nuclear antigen. GADD45A may affect PCNA interaction with some CDK (cell division protein kinase) complexes; stimulates DNA excision repair in vitro and inhibits entry of cells into S phase. GADD45A plays an essential role in active DNA demethylation during terminal osteogenic differentiation of adipose-derived mesenchymal stem cells. GADD45A has Dimer (~28-36 kDa) and Monomer (14-18 kDa) forms (PMID:11498536).

Publications

• Human
• Mouse
• Rat
• Monkey
• Pig

Species	Application	Title
human	WB	Cell Res; DNA damage triggers tubular endoplasmic reticulum extension to promote apoptosis by facilitating ER-mitochondria signaling.; Authors - Pengli Zheng
human	WB	Phytother Res; Activation of endoplasmic reticulum stress by harmine suppresses the growth of esophageal squamous cell carcinoma; Authors - Ronghui Fan
rat	WB	Int J Nanomedicine; TiO2 Nanoparticles Caused DNA Damage in Lung and Extra-Pulmonary Organs Through ROS-Activated FOXO3a Signaling Pathway After Intratracheal Administration in Rats.; Authors - Bin Han
human	WB	Cells; Unraveling the Pathogenetic Mechanisms Underlying the Association between Specific Mitochondrial DNA Haplogroups and Parkinson's Disease; Authors - Min-Yu Lan
human	WB	Sci Rep; Down-regulation of GADD45A enhances chemosensitivity in melanoma.; Authors - Jia Liu
human	WB,IHC	J Exp Clin Cancer Res; Flavagline analog FL3 induces cell cycle arrest in urothelial carcinoma cell of the bladder by inhibiting the Akt/PHB interaction to activate the GADD45α pathway.; Authors - Gangjun Yuan ; KD Validated

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Kamal (Verified Customer) (02-27-2025)

Antibody works well when diluted in 1x TBST.

• Applications: Western Blot
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: Mouse Liver tissue

GADD45A Antibody Western Blot validation (1:1000 dilution) in Mouse Liver tissue (Cat no:13747-1-AP)