GABRD Polyclonal antibody

GABRD Polyclonal Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, ELISA

Conjugate

Unconjugated

Publications

3

Cat no : 15623-1-AP

Synonyms

GABAA receptor delta, GABA A receptor delta, GABA(A) receptor subunit delta, GABRD

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Validation Data Gallery

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  WB analysis using 15623-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 15623-1-AP (GABRD antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 15623-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 15623-1-AP (GABRD antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human brain using 15623-1-AP

  human brain tissue were subjected to SDS PAGE followed by western blot with 15623-1-AP (GABRD antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human kidney using 15623-1-AP

  human kidney tissue were subjected to SDS PAGE followed by western blot with 15623-1-AP (GABRD antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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Tested Applications

• Positive WB detected in: mouse kidney tissue, HeLa cells, human brain tissue, human kidney tissue, rat kidney tissue

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 3 publications below

Product Information

15623-1-AP targets GABRD in WB, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: GABRD fusion protein Ag8064
• Full Name: gamma-aminobutyric acid (GABA) A receptor, delta
• Calculated Molecular Weight: 51 kDa
• Observed Molecular Weight: 51 kDa, 63-66 kDa
• GenBank Accession Number: BC033801
• Gene Symbol: GABRD
• Gene ID (NCBI): 2563
• RRID: AB_2107260
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Gamma-aminobutyric acid (GABA) is the major inhibitory neurotransmitter in the mammalian brain, The GABRD gene encodes the delta subunit of the ligand-gated chloride channel for gamma-aminobutyric acid (GABA).

Publications

Species	Application	Title
human,mouse	WB	Acta Neuropathol Commun; Blood-based CNS regionally and neuronally enriched extracellular vesicles carrying pTau217 for Alzheimer's disease diagnosis and differential diagnosis; Authors - Zhen Guo
mouse,rat	WB	Front Neurosci; Autism-Like Behavior in the Offspring of CYP11A1-Overexpressing Pregnant Rats.; Authors - Tianying Pan
human	WB	Biosci Rep; Validation of the functions and prognostic values of synapse-associated proteins in lower-grade glioma.; Authors - Han Lin