GABPB2 Polyclonal antibody

GABPB2 Polyclonal Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, ELISA

Conjugate

Unconjugated

Publications

1

Cat no : 20870-1-AP

Synonyms

GABP subunit beta 2, GABPB 2, GABPB2

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Validation Data Gallery

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  WB analysis of Jurkat using 20870-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 20870-1-AP (GABPB2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 20870-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 20870-1-AP (GABPB2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HEK-293 using 20870-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 20870-1-AP (GABPB2 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 20870-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 20870-1-AP (GABPB2 antibody) at dilution of 1:100 incubated at room temperature for 1.5 hours.

• 

  WB analysis of A375 using 20870-1-AP

  A375 cells were subjected to SDS PAGE followed by western blot with 20870-1-AP (GABPB2 antibody) at dilution of 1:100 incubated at room temperature for 1.5 hours.

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Tested Applications

• Positive WB detected in: Jurkat cells, A375 cells, HEK-293 cells, HeLa cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 1 publications below
• IP: See 1 publications below

Product Information

The immunogen of 20870-1-AP is GABPB2 Fusion Protein expressed in E. coli.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: GABPB2 fusion protein Ag14933
• Full Name: GA binding protein transcription factor, beta subunit 2
• Calculated Molecular Weight: 448 aa, 49 kDa
• Observed Molecular Weight: 49 kDa
• GenBank Accession Number: BC027033
• Gene Symbol: GABPB2
• Gene ID (NCBI): 126626
• RRID: AB_10734321
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

Species	Application	Title
human	IP	Mol Cell; Argininosuccinate lyase drives activation of mutant TERT promoter in glioblastomas; Authors - Zhumei Shi ; KD Validated