GAB1 Polyclonal antibody

GAB1 Polyclonal Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, rat

Applications

WB, IF, ELISA

Conjugate

Unconjugated

Publications

2

Cat no : 26200-1-AP

Synonyms

GAB1, GRB2 associated binder 1

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Validation Data Gallery

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  WB analysis using 26200-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 26200-1-AP (GAB1 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

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  WB analysis of HEK-293 using 26200-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 26200-1-AP (GAB1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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Tested Applications

• Positive WB detected in: HEK-293 cells, K-562 cells, C6 cells

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:8000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 2 publications below
• IF: See 1 publications below

Product Information

The immunogen of 26200-1-AP is GAB1 Fusion Protein expressed in E. coli.

• Tested Reactivity: human, rat
• Cited Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: GAB1 fusion protein Ag24395
• Full Name: GRB2-associated binding protein 1
• Calculated Molecular Weight: 724 aa, 80 kDa
• Observed Molecular Weight: 110 kDa
• GenBank Accession Number: BC064848
• Gene Symbol: GAB1
• Gene ID (NCBI): 2549
• RRID: AB_2918101
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

Species	Application	Title
human	WB	Anal Chem; Ionic Liquid-Based Extraction System for In-Depth Analysis of Membrane Protein Complexes.; Authors - Hongwei Chu
human	WB,IF	Toxicol Appl Pharmacol; Hexachlorophene, a selective SHP2 inhibitor, suppresses proliferation and metastasis of KRAS-mutant NSCLC cells by inhibiting RAS/MEK/ERK and PI3K/AKT signaling pathways.; Authors - Nai-Jie Fu