G3BP2 Recombinant antibody, PBS Only

G3BP2 Uni-rAb^TM Recombinant Antibody for WB, IHC, IF/ICC, FC (Intra), Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF/ICC, FC (Intra), Indirect ELISA

Conjugate

Unconjugated

CloneNo.

230275G3

Cat no : 82080-6-PBS

Synonyms

G3BP 2, 230275G3, Ras GTPase-activating protein-binding protein 2, GAP SH3 domain-binding protein 2, GAP SH3 domain binding protein 2

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Validation Data Gallery

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  WB analysis using 82080-6-RR (same clone as 82080-6-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 82080-6-RR (G3BP2 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 82080-6-PBS in a different storage buffer formulation.

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  IHC staining of human intrahepatic cholangiocarcinoma using 82080-6-RR (same clone as 82080-6-PBS)

  Immunohistochemical analysis of paraffin-embedded human intrahepatic cholangiocarcinoma tissue slide using 82080-6-RR (G3BP2 antibody) at dilution of 1:1000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 82080-6-PBS in a different storage buffer formulation.

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  IHC staining of human intrahepatic cholangiocarcinoma using 82080-6-RR (same clone as 82080-6-PBS)

  Immunohistochemical analysis of paraffin-embedded human intrahepatic cholangiocarcinoma tissue slide using 82080-6-RR (G3BP2 antibody) at dilution of 1:1000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 82080-6-PBS in a different storage buffer formulation.

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  IHC staining of human ovary cancer using 82080-6-RR (same clone as 82080-6-PBS)

  Immunohistochemical analysis of paraffin-embedded human ovary cancer tissue slide using 82080-6-RR (G3BP2 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 82080-6-PBS in a different storage buffer formulation.

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  IF Staining of HeLa using 82080-6-RR (same clone as 82080-6-PBS)

  Immunofluorescent analysis of (4% PFA) fixed sodium arsenite treated HeLa cells using G3BP2 antibody (82080-6-RR, Clone: 230275G3 ) at dilution of 1:1000 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-phalloidin (red). This data was developed using the same antibody clone with 82080-6-PBS in a different storage buffer formulation.

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  FC experiment of U2OS using 82080-6-RR (same clone as 82080-6-PBS)

  1x10^6 U2OS cells were intracellularly stained with 0.25 ug Anti-Human G3BP2 (82080-6-RR, Clone:230275G3) and APC-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Rabbit IgG control Rabbit PolyAb (30000-0-AP) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 82080-6-PBS in a different storage buffer formulation.

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  Affinity and Kinetic Characterization of 82080-6-RR

  Biolayer interferometry (BLl) kinetic assays of 82080-6-RR against Human G3BP2 were performed. The affinity constant is 4.69 nM.

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Product Information

82080-6-PBS targets G3BP2 in WB, IHC, IF/ICC, FC (Intra), Indirect ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: G3BP2 fusion protein Ag9355
• Full Name: GTPase activating protein (SH3 domain) binding protein 2
• Calculated Molecular Weight: 482aa,54 kDa; 449aa,51 kDa
• Observed Molecular Weight: 65-70 kDa
• GenBank Accession Number: BC011731
• Gene Symbol: G3BP2
• Gene ID (NCBI): 9908
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.

Background Information

Stress granules (SGs) are cytoplasmic mRNA-protein condensates formed in response to cellular stressors, such as oxidative stress, ultraviolet radiation, and viral infection (1). The Ras-GTPase-activating protein-binding proteins (G3BPs), consisting of G3BP1 and G3BP2, are key nucleating factors essential for SG formation. They function to protect RNAs from harmful conditions. G3BP2 is mainly distributed in the cytoplasm and participates in the formation of stress granules, cell differentiation, proliferation, and signal transduction. Accumulating evidence has demonstrated that aberrant expression of G3BP2 contributes to cancer initiation and progression, such as high expression of G3BP2 increasing cell stemness, metastasis and chemoresistance in breast cancer.