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G3BP2 Recombinant antibody, PBS Only

G3BP2 Recombinant Antibody for WB, IF, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human, mouse

Applications

WB, IF, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

230275G3

Cat no : 82080-6-PBS

Synonyms

G3BP 2, G3BP2, KIAA0660

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using G3BP2 Recombinant antibody (82080-6-RR)

    WB analysis using 82080-6-RR (same clone as 82080-6-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 82080-6-RR (G3BP2 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 82080-6-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using G3BP2 Recombinant antibody (82080-6-RR)

    IF Staining of HeLa using 82080-6-RR (same clone as 82080-6-PBS)

    Immunofluorescent analysis of (4% PFA) fixed sodium arsenite treated HeLa cells using G3BP2 antibody (82080-6-RR, Clone: 230275G3 ) at dilution of 1:1000 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-phalloidin (red). This data was developed using the same antibody clone with 82080-6-PBS in a different storage buffer formulation.

  • Affinity experiments of 82080-6-RR

    Affinity experiments of 82080-6-RR

    Biolayer interferometry (BLl) kinetic assays of 82080-6-RR against Human G3BP2 were performed. The affinity constant is 4.69 nM.

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

82080-6-PBS targets G3BP2 in WB, IF, Indirect ELISA applications and shows reactivity with Human, mouse samples.

Tested Reactivity Human, mouse
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen G3BP2 fusion protein Ag9355
Full Name GTPase activating protein (SH3 domain) binding protein 2
Calculated Molecular Weight482aa,54 kDa; 449aa,51 kDa
Observed Molecular Weight65-70 kDa
GenBank Accession NumberBC011731
Gene Symbol G3BP2
Gene ID (NCBI) 9908
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

Stress granules (SGs) are cytoplasmic mRNA-protein condensates formed in response to cellular stressors, such as oxidative stress, ultraviolet radiation, and viral infection (1). The Ras-GTPase-activating protein-binding proteins (G3BPs), consisting of G3BP1 and G3BP2, are key nucleating factors essential for SG formation. They function to protect RNAs from harmful conditions. G3BP2 is mainly distributed in the cytoplasm and participates in the formation of stress granules, cell differentiation, proliferation, and signal transduction. Accumulating evidence has demonstrated that aberrant expression of G3BP2 contributes to cancer initiation and progression, such as high expression of G3BP2 increasing cell stemness, metastasis and chemoresistance in breast cancer.