G3BP2 Recombinant antibody, PBS Only (Capture)

G3BP2 Recombinant Antibody for WB, IF, Indirect ELISA, Cytometric bead array

Host / Isotype

Rabbit / IgG

Reactivity

Human

Applications

WB, IF, Indirect ELISA, Cytometric bead array

Conjugate

Unconjugated

CloneNo.

230275D6

Cat no : 82080-4-PBS

Synonyms

G3BP 2, G3BP2, KIAA0660

---

Validation Data Gallery

• 

  WB analysis using 82080-4-RR (same clone as 82080-4-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 82080-4-RR (G3BP2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 82080-4-PBS in a different storage buffer formulation.

• 

  IF Staining of HeLa using 82080-4-RR (same clone as 82080-4-PBS)

  Immunofluorescent analysis of (4% PFA) fixed sodium arsenite treated HeLa cells using G3BP2 antibody (82080-4-RR, Clone: 230275D6 ) at dilution of 1:1000 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 82080-4-PBS in a different storage buffer formulation.

• 

  Cytometric bead array standard curve of MP00090-1

  Cytometric bead array standard curve ofMP00090-1, G3BP2 Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 82080-4-PBS. Detection antibody: 82080-3-PBS. Standard: Ag9355. Range: 0.625-20 ng/mL.

• 

  Cytometric bead array standard curve of MP00090-2

  Cytometric bead array standard curve ofMP00090-2, G3BP2 Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 82080-4-PBS. Detection antibody: 82080-5-PBS. Standard: Ag9355. Range: 0.625-20 ng/mL.

• 

  Cytometric bead array standard curve of MP00090-3

  Cytometric bead array standard curve ofMP00090-3, G3BP2 Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 82080-4-PBS. Detection antibody: 82080-2-PBS. Standard: Ag9355. Range: 0.625-20 ng/mL.

• 

  Affinity experiments of 82080-4-RR

  Biolayer interferometry (BLl) kinetic assays of 82080-4-RR against Human G3BP2 were performed. The affinity constant is 59.6 nM.

---

Recommended dilution

Application	Dilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

82080-4-PBS targets G3BP2 as part of a matched antibody pair:

MP00090-1: 82080-4-PBS capture and 82080-3-PBS detection (validated in Cytometric bead array)

MP00090-2: 82080-4-PBS capture and 82080-5-PBS detection (validated in Cytometric bead array)

MP00090-3: 82080-4-PBS capture and 82080-2-PBS detection (validated in Cytometric bead array)

Unconjugated rabbit recombinant monoclonal antibody in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

• Tested Reactivity: Human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: G3BP2 fusion protein Ag9355
• Full Name: GTPase activating protein (SH3 domain) binding protein 2
• Calculated Molecular Weight: 482aa,54 kDa; 449aa,51 kDa
• GenBank Accession Number: BC011731
• Gene Symbol: G3BP2
• Gene ID (NCBI): 9908
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: 100% PBS pH 7.3
• Storage Conditions: Store at -80°C.