G3BP2 Monoclonal antibody

G3BP2 Monoclonal Antibody for WB, IF/ICC, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human, rat

Applications

WB, IF/ICC, ELISA

Conjugate

Unconjugated

CloneNo.

2E5G3

Cat no : 68580-1-Ig

Synonyms

G3BP 2, G3BP2, KIAA0660

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Validation Data Gallery

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  WB analysis of SCaBER using 68580-1-Ig

  SCaBER cells were subjected to SDS PAGE followed by western blot with 68580-1-Ig (G3BP2 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 68580-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 68580-1-Ig (G3BP2 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control.

• 

  WB analysis of A549 using 68580-1-Ig

  A549 cells were subjected to SDS PAGE followed by western blot with 68580-1-Ig (G3BP2 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of rat brain using 68580-1-Ig

  rat brain tissue were subjected to SDS PAGE followed by western blot with 68580-1-Ig (G3BP2 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HCT 116 using 68580-1-Ig

  HCT 116 cells were subjected to SDS PAGE followed by western blot with 68580-1-Ig (G3BP2 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 68580-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 68580-1-Ig (G3BP2 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control.

• 

  IF Staining of HeLa using 68580-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed sodium arsenite treated HeLa cells using G3BP2 antibody (68580-1-Ig, Clone: 2E5G3 ) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L), CL594-phalloidin (red).

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Tested Applications

• Positive WB detected in: HeLa cells, SCaBER cells, rat brain tissue, HCT 116 cells, HepG2 cells, A549 cells, HT-29 cells, K-562 cells, MOLT-4 cells
• Positive IF/ICC detected in: sodium arsenite treated HeLa cells

Recommended dilution

• Western Blot (WB): WB : 1:5000-1:50000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:400-1:1600

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

68580-1-Ig targets G3BP2 in WB, IF/ICC, ELISA applications and shows reactivity with Human, rat samples.

• Tested Reactivity: Human, rat
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen: G3BP2 fusion protein Ag9222
• Full Name: GTPase activating protein (SH3 domain) binding protein 2
• Calculated Molecular Weight: 482aa,54 kDa; 449aa,51 kDa
• Observed Molecular Weight: 54 kDa
• GenBank Accession Number: BC011731
• Gene Symbol: G3BP2
• Gene ID (NCBI): 9908
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein G purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Stress granules (SGs) are cytoplasmic mRNA-protein condensates formed in response to cellular stressors, such as oxidative stress, ultraviolet radiation, and viral infection (1). The Ras-GTPase-activating protein-binding proteins (G3BPs), consisting of G3BP1 and G3BP2, are key nucleating factors essential for SG formation. They function to protect RNAs from harmful conditions. G3BP2 is mainly distributed in the cytoplasm and participates in the formation of stress granules, cell differentiation, proliferation, and signal transduction. Accumulating evidence has demonstrated that aberrant expression of G3BP2 contributes to cancer initiation and progression, such as high expression of G3BP2 increasing cell stemness, metastasis and chemoresistance in breast cancer.