G3BP1 Recombinant antibody

G3BP1 Uni-rAb^TM Recombinant Antibody for IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

IF/ICC, ELISA

Conjugate

Unconjugated

CloneNo.

7F14

Cat no : 80777-1-RR

Synonyms

G3BP-1, G3BP 1, G3BP, EC:3.6.4.12, ATP-dependent DNA helicase VIII

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Validation Data Gallery

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  IF Staining of HeLa using 80777-1-RR

  Immunofluorescent analysis of (4% PFA) fixed sodium arsenite treated HeLa cells using G3BP1 antibody (80777-1-RR, Clone: 7F14 ) at dilution of 1:1400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red).

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  Affinity and Kinetic Characterization of 80777-1-RR

  Biolayer interferometry (BLl) kinetic assays of 80777-1-RR against Human G3BP1 were performed. The affinity constant is 7.07 nM.

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Tested Applications

• Positive IF/ICC detected in: sodium arsenite treated HeLa cells

Recommended dilution

• Immunofluorescence (IF)/ICC: IF/ICC : 1:500-1:2000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

80777-1-RR targets G3BP1 in IF/ICC, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: G3BP1 fusion protein Ag3728
• Full Name: GTPase activating protein (SH3 domain) binding protein 1
• Calculated Molecular Weight: 466 aa, 52 kDa
• GenBank Accession Number: BC006997
• Gene Symbol: G3BP1
• Gene ID (NCBI): 10146
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

GAP SH3 Binding Protein 1 (G3BP1), also named as G3BP, is an effector of stress granule (SG) assembly. SG biology plays an important role in the pathophysiology of TDP-43 in ALS and FTLD-U. G3BP1 can be used as a marker of SG. It has been shown to function downstream of Ras and play a role in RNA metabolism, signal transduction, and proliferation. G3BP1 is a ubiquitously expressed protein that localizes to the cytoplasm in proliferating cells and to the nucleus in non-proliferating cells. G3BP1 has recently been implicated in cancer biology.