G-CSFR Polyclonal antibody

G-CSFR Polyclonal Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human and More (1)

Applications

WB, IHC, IF, ELISA

Conjugate

Unconjugated

Publications

3

Cat no : 18310-1-AP

Synonyms

CD114, CSF3R, G CSF R, G CSF receptor, GCSFR, G-CSFR, G-CSF-R

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Validation Data Gallery

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  WB analysis of human placenta using 18310-1-AP

  human placenta tissue were subjected to SDS PAGE followed by western blot with 18310-1-AP (G-CSFR antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human placenta using 18310-1-AP

  human placenta tissue were subjected to SDS PAGE followed by western blot with 18310-1-AP (G-CSFR antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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Tested Applications

• Positive WB detected in: human placenta tissue

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 1 publications below
• WB: See 3 publications below
• IHC: See 1 publications below
• IF: See 1 publications below

Product Information

18310-1-AP targets G-CSFR in WB, IHC, IF, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: Peptide
• Full Name: colony stimulating factor 3 receptor (granulocyte)
• Calculated Molecular Weight: 95 kDa
• Observed Molecular Weight: 85 kDa
• GenBank Accession Number: BC053585
• Gene Symbol: G-CSFR
• Gene ID (NCBI): 1441
• RRID: AB_2083865
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

G-CSF-R, also named as CSF3R, CD114 and GCSFR is the receptor for colony stimulating factor 3, a cytokine that controls the production, differentiation, and function of granulocytes. It is a 130 kD to 150 kD glycoprotein single chain receptor which binds and activated by GCSF. In addition it may function in some adhesion or recognition events at the cell surface. It has been classified as a member of the hematopoietic (cytokine) receptor family, cytokine receptor class I, or the gp 130 related cytokine receptor family (although it does not apparently bind to gp 130). GCSF receptors can be found on neutrophils, myeloid leukemia cells that respond to GCSF, bone marrow cells of neutrophilic granulocyte lineage, and on placental trophoblasts. In addition, a soluble form is also expressed. Mutations in this gene are a cause of Kostmann syndrome, also known as severe congenital neutropenia.

Publications

Species	Application	Title
mouse	WB	Mol Metab; Ovarian cancer modulates the immunosuppressive function of CD11b+Gr1+ myeloid cells via glutamine metabolism.; Authors - Mary P Udumula
human,mouse	WB,IHC,IF	Int Immunopharmacol; CSF3 aggravates acute exacerbation of pulmonary fibrosis by disrupting alveolar epithelial barrier integrity; Authors - Bingnan Guo ; KD Validated
human	WB	J Cell Physiol; The overexpression of GRASP might inhibit cell proliferation and invasion in hepatocellular carcinoma.; Authors - Yang Cheng