Frizzled 9 Monoclonal antibody, PBS Only

Frizzled 9 Monoclonal Antibody for WB, IF, IHC, Indirect ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human

Applications

WB, IF, IHC, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

1G9G4

Cat no : 67023-1-PBS

Synonyms

CD349, Frizzled 9, Fz 9, FZD3, FZD9, FzE6, hFz9

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Validation Data Gallery

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  WB analysis of A549 using 67023-1-Ig (same clone as 67023-1-PBS)

  A549 cells were subjected to SDS PAGE followed by western blot with 67023-1-Ig (Frizzled 9 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67023-1-PBS in a different storage buffer formulation.

• 

  WB analysis of HeLa using 67023-1-Ig (same clone as 67023-1-PBS)

  HeLa cells were subjected to SDS PAGE followed by western blot with 67023-1-Ig (Frizzled 9 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67023-1-PBS in a different storage buffer formulation.

• 

  WB analysis of HUVEC using 67023-1-Ig (same clone as 67023-1-PBS)

  HUVEC cells were subjected to SDS PAGE followed by western blot with 67023-1-Ig (Frizzled 9 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67023-1-PBS in a different storage buffer formulation.

• 

  WB analysis of NCI-H1299 using 67023-1-Ig (same clone as 67023-1-PBS)

  NCI-H1299 cells were subjected to SDS PAGE followed by western blot with 67023-1-Ig (Frizzled 9 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67023-1-PBS in a different storage buffer formulation.

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  WB analysis of fetal human brain using 67023-1-Ig (same clone as 67023-1-PBS)

  fetal human brain tissue were subjected to SDS PAGE followed by western blot with 67023-1-Ig (Frizzled 9 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67023-1-PBS in a different storage buffer formulation.

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  IHC staining of human gliomas using 67023-1-Ig (same clone as 67023-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 67023-1-Ig (Frizzled 9 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67023-1-PBS in a different storage buffer formulation.

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  IHC staining of human gliomas using 67023-1-Ig (same clone as 67023-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 67023-1-Ig (Frizzled 9 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67023-1-PBS in a different storage buffer formulation.

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  IF Staining of HEK-293 using 67023-1-Ig (same clone as 67023-1-PBS)

  Immunofluorescent analysis of (-20°C Methanol) fixed HEK-293 cells using Frizzled 9 antibody (67023-1-Ig, Clone: 1G9G4 ) at dilution of 1:800 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 67023-1-PBS in a different storage buffer formulation.

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Recommended dilution

Application	Dilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

67023-1-PBS targets Frizzled 9 in WB, IF, IHC, Indirect ELISA applications and shows reactivity with Human samples.

• Tested Reactivity: Human
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen: Frizzled 9 fusion protein Ag4971
• Full Name: frizzled homolog 9 (Drosophila)
• Calculated Molecular Weight: 591 aa, 64 kDa
• Observed Molecular Weight: 64-70 kDa
• GenBank Accession Number: BC026333
• Gene Symbol: FZD9
• Gene ID (NCBI): 8326
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein G purification
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.