Flotillin 1 Monoclonal antibody, PBS Only

Flotillin 1 Monoclonal Antibody for WB, FC (Intra), Indirect ELISA

Host / Isotype

Mouse / IgG2b

Reactivity

human, mouse, rat, pig, rabbit

Applications

WB, FC (Intra), Indirect ELISA

Conjugate

Unconjugated

CloneNo.

1D6C6

Cat no : 67968-1-PBS

Synonyms

FLOT1, Flotillin-1, 1D6C6

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Validation Data Gallery

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  WB analysis using 67968-1-Ig (same clone as 67968-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 67968-1-Ig (Flotillin 1 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67968-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human ovary tumor using 67968-1-Ig (same clone as 67968-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 67968-1-Ig (Flotillin 1 antibody) at dilution of 1:4000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67968-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human stomach cancer using 67968-1-Ig (same clone as 67968-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 67968-1-Ig (Flotillin 1 antibody) at dilution of 1:4000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67968-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human stomach cancer using 67968-1-Ig (same clone as 67968-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 67968-1-Ig (Flotillin 1 antibody) at dilution of 1:4000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67968-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human ovary tumor using 67968-1-Ig (same clone as 67968-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 67968-1-Ig (Flotillin 1 antibody) at dilution of 1:4000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67968-1-PBS in a different storage buffer formulation.

• 

  FC experiment of Raji using 67968-1-Ig (same clone as 67968-1-PBS)

  1x10^6 Raji cells were intracellularly stained with 0.2 ug Flotillin 1 Monoclonal antibody (67968-1-Ig, Clone:1D6C6) and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1)(red), or 0.2 ug Mouse IgG2b isotype control Mouse McAb (66360-3-Ig, Clone: 11B8C4) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 67968-1-PBS in a different storage buffer formulation.

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Product Information

67968-1-PBS targets Flotillin 1 in WB, FC (Intra), Indirect ELISA applications and shows reactivity with human, mouse, rat, pig, rabbit samples.

• Tested Reactivity: human, mouse, rat, pig, rabbit
• Host / Isotype: Mouse / IgG2b
• Class: Monoclonal
• Type: Antibody
• Immunogen: Flotillin 1 fusion protein Ag8694
• Full Name: flotillin 1
• Calculated Molecular Weight: 47 kDa
• Observed Molecular Weight: 47 kDa
• GenBank Accession Number: BC001146
• Gene Symbol: Flotillin 1
• Gene ID (NCBI): 10211
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.

Background Information

Flotillins are membrane-associated proteins that contain two homologous isoforms, flotillin 1 (FLOT1) and flotillin 2 (FLOT2). They are highly conserved and widely expressed proteins that commonly used as markers of lipid rafts. Flotillins are involved in various signaling pathways, cell adhesion, membrane trafficking and axonal growth.