Filamin B Recombinant antibody

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  WB analysis using 85202-3-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 85202-3-RR (Filamin B antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

Filamin B {ptg:Brand} Recombinant Antibody for WB, ELISA

Cat No. 85202-3-RR

Clone No.242275G9

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, ELISA

Synonyms

FLNB, Filamin-3, Beta-filamin, Actin-binding-like protein, ABP-280 homolog

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Tested Applications

• Positive WB detected in: HeLa cells, U2OS cells, HEK-293T cells, A431 cells, C2C12 cells, NIH/3T3 cells, human placenta tissue

Recommended dilution

• Western Blot (WB): WB : 1:5000-1:50000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

85202-3-RR targets Filamin B in WB, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: Peptide
• Full Name: filamin B, beta (actin binding protein 278)
• Calculated Molecular Weight: 278 kDa
• Observed Molecular Weight: 280-300 kDa
• GenBank Accession Number: NM_001457
• Gene Symbol: FLNB
• Gene ID (NCBI): 2317
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: O75369
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

FLNB, also named as FLN1L, FLN3, TABP, TAP and Fh1, belongs to the filamin family. FLNB connects cell membrane constituents to the actin cytoskeleton. It may promote orthogonal branching of actin filaments and links actin filaments to membrane glycoproteins. It is an anchors various transmembrane proteins to the actin cytoskeleton. Interaction with FLNA, FLNB may allow neuroblast migration from the ventricular zone into the cortical plate. Various interactions and localizations of isoforms affect myotube morphology and myogenesis.