Fibroblast activation protein alpha Polyclonal antibody

Fibroblast activation protein alpha Polyclonal Antibody for IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

IHC, ELISA

Conjugate

Unconjugated

Cat no : 27596-1-AP

Synonyms

FAP, FAPA, EC:3.4.21.26, EC:3.4.21.-, EC:3.4.14.5

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Validation Data Gallery

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  IHC staining of human breast cancer using 27596-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 27596-1-AP (Fibroblast activation protein alpha antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human breast cancer using 27596-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 27596-1-AP (Fibroblast activation protein alpha antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse liver using 27596-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 27596-1-AP (Fibroblast activation protein alpha antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse liver using 27596-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 27596-1-AP (Fibroblast activation protein alpha antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

• Positive IHC detected in: human breast cancer tissue, mouse liver; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

27596-1-AP targets Fibroblast activation protein alpha in IHC, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: Fibroblast activation protein alpha fusion protein Ag26695
• Full Name: fibroblast activation protein, alpha
• Calculated Molecular Weight: 760 aa, 88 kDa
• GenBank Accession Number: BC026250
• Gene Symbol: FAP
• Gene ID (NCBI): 2191
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.