Fibrillin 1 Polyclonal antibody, PBS Only

Fibrillin 1 Polyclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, IF/ICC, Indirect ELISA

Conjugate

Unconjugated

Cat no : 29425-1-PBS

Synonyms

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Validation Data Gallery

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  WB analysis using 29425-1-AP (same clone as 29425-1-PBS)

  HeLa cells were subjected to Tris-acetate gel system followed by western blot with 29425-1-AP (Fibrillin 1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 29425-1-PBS in a different storage buffer formulation.

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  IHC staining of human endometrial cancer using 29425-1-AP (same clone as 29425-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human endometrial cancer tissue slide using 29425-1-AP (Fibrillin 1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 29425-1-PBS in a different storage buffer formulation.

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  IHC staining of human endometrial cancer using 29425-1-AP (same clone as 29425-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human endometrial cancer tissue slide using 29425-1-AP (Fibrillin 1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 29425-1-PBS in a different storage buffer formulation.

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  IHC staining of human colon using 29425-1-AP (same clone as 29425-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 29425-1-AP (Fibrillin 1 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 29425-1-PBS in a different storage buffer formulation.

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  IF Staining of A431 using 29425-1-AP (same clone as 29425-1-PBS)

  Immunofluorescent analysis of (-20°C Ethanol) fixed A431 cells using Fibrillin 1 antibody (29425-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-phalloidin (red). This data was developed using the same antibody clone with 29425-1-PBS in a different storage buffer formulation.

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Product Information

29425-1-PBS targets Fibrillin 1 in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: Fibrillin 1 fusion protein Ag30283
• Full Name: fibrillin 1
• Observed Molecular Weight: 350 kDa
• GenBank Accession Number: BC146854
• Gene Symbol: Fibrillin 1
• Gene ID (NCBI): 2200
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.

Background Information

Fibrillin 1 is a 350 kDa large extracellular matrix glycoprotein that formed thread-like microfibril filaments, which provided structural support for tissues, formed elastic fibers in the skin and vessels, regulated the availability of the transforming growth factor beta (TGF-β) protein, and modulated the microenvironment. Fibrillin 1 contains a large number of cysteine-rich epidermal growth factor (EGF)- like domains, most capable of binding calcium ions (cbEGF-like), and a few motifs homologous to the binding protein for transforming growth factor beta (TGFβ1-BP)- like module (PMID: 29220879, PMID: 35234370).