Tested Applications
Positive WB detected in | MC/9 cells |
Recommended dilution
Application | Dilution |
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Western Blot (WB) | WB : 1:5000-1:50000 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
Product Information
84816-4-RR targets FceR1a in WB, ELISA applications and shows reactivity with mouse samples.
Tested Reactivity | mouse |
Host / Isotype | Rabbit / IgG |
Class | Recombinant |
Type | Antibody |
Immunogen | Recombinant protein Predict reactive species |
Full Name | Fc receptor, IgE, high affinity I, alpha polypeptide |
Calculated Molecular Weight | 29 kDa |
Observed Molecular Weight | 42 kDa |
GenBank Accession Number | NM_010184.2 |
Gene Symbol | FceR1a |
Gene ID (NCBI) | 14125 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Protein A purification |
UNIPROT ID | P20489 |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
Fc epsilon Receptor I alpha (FceR1a) is a single-pass type I membrane protein that contains 2 immunoglobulin-like domains. FceR1a forms a tetrameric complex with one beta and two gamma subunits (PMID: 19406478). FceRI is the high-affinity receptor for the Fc region of immunoglobulin E (IgE) (PMID: 1532373). It is expressed on mast cells and basophils and plays a central role in the allergic response (PMID: 2148316).
Protocols
Product Specific Protocols | |
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WB protocol for FceR1a antibody 84816-4-RR | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |