Filter: AllWBIHCIF-PIF/ICCFC (Intra)

WB analysis of SH-SY5Y using 66321-1-Ig (same clone as 66321-1-PBS)
SH-SY5Y cells were subjected to SDS PAGE followed by western blot with 66321-1-Ig (Fascin Antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66321-1-PBS in a different storage buffer formulation.
WB analysis of K-562 using 66321-1-Ig (same clone as 66321-1-PBS)
K-562 cells were subjected to SDS PAGE followed by western blot with 66321-1-Ig (Fascin Antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66321-1-PBS in a different storage buffer formulation.
WB analysis of HeLa using 66321-1-Ig (same clone as 66321-1-PBS)
HeLa cells were subjected to SDS PAGE followed by western blot with 66321-1-Ig (Fascin Antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66321-1-PBS in a different storage buffer formulation.
IHC staining of human tonsillitis using 66321-1-Ig (same clone as 66321-1-PBS)
Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 66321-1-Ig (Fascin Antibody) at dilution of 1:600 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66321-1-PBS in a different storage buffer formulation.
IHC staining of human tonsillitis using 66321-1-Ig (same clone as 66321-1-PBS)
Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 66321-1-Ig (Fascin Antibody) at dilution of 1:600 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66321-1-PBS in a different storage buffer formulation.
IHC staining of human tonsillitis using 66321-1-Ig (same clone as 66321-1-PBS)
Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 66321-1-Ig (Fascin antibody) at dilution of 1:8000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66321-1-PBS in a different storage buffer formulation.
IHC staining of human pancreas cancer using 66321-1-Ig (same clone as 66321-1-PBS)
Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 66321-1-Ig (Fascin Antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66321-1-PBS in a different storage buffer formulation.
IHC staining of human skin cancer using 66321-1-Ig (same clone as 66321-1-PBS)
Immunohistochemical analysis of paraffin-embedded human skin cancer tissue slide using 66321-1-Ig (Fascin Antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66321-1-PBS in a different storage buffer formulation.
IHC staining of human skin cancer using 66321-1-Ig (same clone as 66321-1-PBS)
Immunohistochemical analysis of paraffin-embedded human skin cancer tissue slide using 66321-1-Ig (Fascin Antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66321-1-PBS in a different storage buffer formulation.
IHC staining of human pancreas cancer using 66321-1-Ig (same clone as 66321-1-PBS)
Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 66321-1-Ig (Fascin Antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66321-1-PBS in a different storage buffer formulation.
IF Staining of human tonsillitis using 66321-1-Ig (same clone as 66321-1-PBS)
Immunofluorescent analysis of (4% PFA) fixed human tonsillitis tissue using 66321-1-Ig (Fascin antibody) at dilution of 1:100 and CoraLite488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66321-1-PBS in a different storage buffer formulation.
IF Staining of human tonsillitis using 66321-1-Ig (same clone as 66321-1-PBS)
Immunofluorescent analysis of (4% PFA) fixed human tonsillitis tissue using Fascin antibody (66321-1-Ig, Clone: 1B5G3 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66321-1-PBS in a different storage buffer formulation.
IF Staining of SH-SY5Y using 66321-1-Ig (same clone as 66321-1-PBS)
Immunofluorescent analysis of (-20℃ Ethanol ) fixed SH-SY5Y cells using 66321-1-Ig(Fascin antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66321-1-PBS in a different storage buffer formulation.
FC experiment of SH-SY5Y using 66321-1-Ig (same clone as 66321-1-PBS)
1x10^6 SH-SY5Y cells were intracellularly stained with 0.4 ug Fascin Monoclonal antibody (66321-1-Ig, Clone:1B5G3) and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) (SA00013-1)(red), or 0.4 ug Mouse IgG2a isotype control Mouse McAb (66360-2-Ig, Clone: 11A1B2) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 66321-1-PBS in a different storage buffer formulation.
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1x10^6 SH-SY5Y cells were intracellularly stained with 0.4 ug Fascin Monoclonal antibody (66321-1-Ig, Clone:1B5G3) and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) (SA00013-1)(red), or 0.4 ug Mouse IgG2a isotype control Mouse McAb (66360-2-Ig, Clone: 11A1B2) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 66321-1-PBS in a different storage buffer formulation.

Featured Product
KD/KO Validated

Fascin Monoclonal antibody, PBS Only

Fascin Monoclonal Antibody for WB, IHC, IF/ICC, IF-P, FC (Intra), Indirect ELISA

Cat No. 66321-1-PBS

Clone No.1B5G3

Review Product

Host / Isotype

Mouse / IgG2a

Reactivity

human

Applications

WB, IHC, IF/ICC, IF-P, FC (Intra), Indirect ELISA

Synonyms

FSCN1, Fascin 1,Fascin, FAN1, 55 kDa actin-bundling protein, 55 kDa actin bundling protein

Formulation: PBS Only

PBS and Azide

PBS Only

Conjugate: Unconjugated

Size/Concentration:

-/ -

Freight/Packing: -

Quantity

- +

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Overview

Tested Applications
Product Information

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MSDS SDS

Product Information

66321-1-PBS targets Fascin in WB, IHC, IF/ICC, IF-P, FC (Intra), Indirect ELISA applications and shows reactivity with human samples.

Tested Reactivity	human
Host / Isotype	Mouse / IgG2a
Class	Monoclonal
Type	Antibody
Immunogen	Fascin fusion protein Ag6074 Predict reactive species
Full Name	fascin homolog 1, actin-bundling protein (Strongylocentrotus purpuratus)
Calculated Molecular Weight	55 kDa
Observed Molecular Weight	55 kDa
GenBank Accession Number	BC000521
Gene Symbol	Fascin
Gene ID (NCBI)	6624
ENSEMBL Gene ID	ENSG00000075618
RRID	AB_2881701
Conjugate	Unconjugated
Form	Liquid
Purification Method	Protein A purification
UNIPROT ID	Q16658
Storage Buffer	PBS Only
Storage Conditions	Store at -80°C.

Background Information

Fascin is a highly conserved and ubiquitous actin cross-linking protein that has a major function in cell motility and adhesion. Fascin localizes to a number of highly dynamic cellular structures that require strong mechanical support which include stress fibers and cellular protrusions such as microvilli, microspikes and lamellipodia. Fascin is up-regulated in many human carcinomas. It's a marker for Reed-Sternberg cells in Hodgkin's disease; it is also a highly selective marker for dendritic cells of lymphoid tissues.