Fas/CD95 Recombinant antibody, PBS Only

Fas/CD95 Recombinant Antibody for IF, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human

Applications

IF, ELISA

Conjugate

Unconjugated

CloneNo.

240340E11

Cat no : 83400-5-PBS

Synonyms

ALPS1A, APO 1, Apo 1 antigen, APT1, CD95, FAS, FAS1, FASLG receptor, FASTM, TNFRSF6

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Validation Data Gallery

• 

  IF Staining of Raji using 83400-5-RR (same clone as 83400-5-PBS)

  Immunofluorescent analysis of (4% PFA) fixed Raji cells using Fas/CD95 antibody (83400-5-RR, Clone: 240340E11 ) at dilution of 1:250 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) (SA00013-1). This data was developed using the same antibody clone with 83400-5-PBS in a different storage buffer formulation.

• 

  IF Staining of Raji using 83400-5-RR (same clone as 83400-5-PBS)

  Immunofluorescent analysis of (4% PFA) fixed Raji cells using Fas/CD95 antibody (83400-5-RR, Clone: 240340E11 ) at dilution of 1:250 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) (SA00013-1). This data was developed using the same antibody clone with 83400-5-PBS in a different storage buffer formulation.

• 

  IF Staining of Jurkat using 83400-5-RR (same clone as 83400-5-PBS)

  Immunofluorescent analysis of (4% PFA) fixed Jurkat cells using Fas/CD95 antibody (83400-5-RR, Clone: 240340E11 ) at dilution of 1:250 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) (SA00013-1). This data was developed using the same antibody clone with 83400-5-PBS in a different storage buffer formulation.

• 

  IF Staining of Jurkat using 83400-5-RR (same clone as 83400-5-PBS)

  Immunofluorescent analysis of (4% PFA) fixed Jurkat cells using Fas/CD95 antibody (83400-5-RR, Clone: 240340E11 ) at dilution of 1:250 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) (SA00013-1). This data was developed using the same antibody clone with 83400-5-PBS in a different storage buffer formulation.

• 

  Affinity experiments of 83400-5-RR

  Biolayer interferometry (BLl) kinetic assays of 83400-5-RR against Human Fas/CD95 were performed. The affinity constant is 0.927 nM.

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Recommended dilution

Application	Dilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

The immunogen of 83400-5-PBS is Fas/CD95 Fusion Protein expressed in E. coli.

• Tested Reactivity: Human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: Fusion Protein
• Full Name: Fas (TNF receptor superfamily, member 6)
• Calculated Molecular Weight: 38 kDa
• GenBank Accession Number: NM_000043.6
• Gene Symbol: CD95
• Gene ID (NCBI): 355
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purfication
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.