Factor XII Monoclonal antibody, PBS Only

Factor XII Monoclonal Antibody for WB, IHC, IF-P, Indirect ELISA

Host / Isotype

Mouse / IgG2b

Reactivity

human, mouse

Applications

WB, IHC, IF-P, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

2E2F11

Cat no : 66089-1-PBS

Synonyms

F12, Factor 12, EC:3.4.21.38, Coagulation factor XII, Beta-factor XIIa part 1

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Validation Data Gallery

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  WB analysis of K-562 using 66089-1-Ig (same clone as 66089-1-PBS)

  K-562 cells were subjected to SDS PAGE followed by western blot with 66089-1-Ig (Factor XII antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66089-1-PBS in a different storage buffer formulation.

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  IHC staining of human liver using 66089-1-Ig (same clone as 66089-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 66089-1-Ig (Factor XII antibody at dilution of 1:200 (under 10x lens). This data was developed using the same antibody clone with 66089-1-PBS in a different storage buffer formulation.

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  IHC staining of human liver using 66089-1-Ig (same clone as 66089-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 66089-1-Ig (Factor XII antibody at dilution of 1:200 (under 40x lens). This data was developed using the same antibody clone with 66089-1-PBS in a different storage buffer formulation.

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  IHC staining of human kidney using 66089-1-Ig (same clone as 66089-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 66089-1-Ig (Factor XII antibody at dilution of 1:200 (under 10x lens). This data was developed using the same antibody clone with 66089-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human kidney using 66089-1-Ig (same clone as 66089-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 66089-1-Ig (Factor XII antibody at dilution of 1:200 (under 40x lens). This data was developed using the same antibody clone with 66089-1-PBS in a different storage buffer formulation.

• 

  IF Staining of mouse liver using 66089-1-Ig (same clone as 66089-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed mouse liver tissue using Factor XII antibody (66089-1-Ig, Clone: 2E2F11 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66089-1-PBS in a different storage buffer formulation.

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  IF Staining of HepG2 using 66089-1-Ig (same clone as 66089-1-PBS)

  Immunofluorescent analysis of HepG2 cells, using F12 antibody 66089-1-lg at 1:25 dilution and Rhodamine-labeled goat anti-mouse IgG (red). This data was developed using the same antibody clone with 66089-1-PBS in a different storage buffer formulation.

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Product Information

66089-1-PBS targets Factor XII in WB, IHC, IF-P, Indirect ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Host / Isotype: Mouse / IgG2b
• Class: Monoclonal
• Type: Antibody
• Immunogen: Factor XII fusion protein Ag3401
• Full Name: coagulation factor XII (Hageman factor)
• Calculated Molecular Weight: 615 aa, 68 kDa
• Observed Molecular Weight: 28 kDa
• GenBank Accession Number: BC012390
• Gene Symbol: Factor XII
• Gene ID (NCBI): 2161
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.

Background Information

Factor XII (F XII, Hageman factor) is a 80 kDa, single chain glycoprotein that circulates in blood as an inactive zymogen. F XII plays an important role in blood coagulation, fibrinolysis, and kinin generation. Factor XII is activated by kallikrein in alpha-factor XIIa, which is further converted by trypsin into beta-factor XIIa. Alpha-factor XIIa is composed of a 52 kDa NH2-terminal heavy chain, called coagulation factor XIIa heavy chain, and a 28 kDa COOH-terminal light chain, called coagulation factor XIIa light chain, connected by a disulfide bond. Beta-factor XIIa is composed of 2 chains linked by a disulfide bond, an N-terminal nonapeptide, called beta-factor XIIa part 1, and coagulation factor XIIa light chain, also known in this context as beta-factor XIIa part 2.