Frizzled 8 Polyclonal antibody

Frizzled 8 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 55093-1-AP

Synonyms

Frizzled 8, FZ 8, FZD8, hFZ8



Tested Applications

Positive WB detected inHeLa cells, mouse embryo tissue, mouse heart tissue, mouse lung tissue
Positive IHC detected inhuman pancreas cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WBSee 1 publications below

Product Information

55093-1-AP targets Frizzled 8 in WB, IHC, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Cited Reactivityhuman
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Peptide
Full Name frizzled homolog 8 (Drosophila)
Calculated Molecular Weight 73 kDa
Observed Molecular Weight 70-73 kDa
GenBank Accession NumberNM_031866
Gene Symbol Frizzled 8
Gene ID (NCBI) 8325
RRIDAB_2881267
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer 0.1M NaHCO3, 0.1M glycine, 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
WB protocol for Frizzled 8 antibody 55093-1-APDownload protocol
IHC protocol for Frizzled 8 antibody 55093-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Theranostics

Alkaline Phosphatase Controls Lineage Switching of Mesenchymal Stem Cells by Regulating the LRP6/GSK3β Complex in Hypophosphatasia.

Authors - Wenjia Liu