Frizzled 8 Polyclonal antibody

Frizzled 8 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Publications

1

Cat no : 55093-1-AP

Synonyms

Frizzled 8, FZ 8, FZD8, hFZ8

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Validation Data Gallery

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  WB analysis of HeLa using 55093-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 55093-1-AP (Frizzled 8 antibody at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse embryo using 55093-1-AP

  mouse embryo tissue were subjected to SDS PAGE followed by western blot with 55093-1-AP (Frizzled 8 antibody at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse lung using 55093-1-AP

  mouse lung tissue were subjected to SDS PAGE followed by western blot with 55093-1-AP (Frizzled 8 antibody) at dilution of 1:200 incubated at room temperature for 1.5 hours.

• 

  WB analysis of multi-cells/tissue using 55093-1-AP

  WB result of 55093-1-AP (Frizzled 8 antibody).

• 

  WB analysis of mouse heart using 55093-1-AP

  mouse heart tissue were subjected to SDS PAGE followed by western blot with 55093-1-AP (Frizzled 8 antibody) at dilution of 1:200 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human pancreas cancer using 55093-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 55093-1-AP (Frizzled 8 antibody at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human pancreas cancer using 55093-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 55093-1-AP (Frizzled 8 antibody at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

• Positive WB detected in: HeLa cells, mouse embryo tissue, mouse heart tissue, mouse lung tissue
• Positive IHC detected in: human pancreas cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 1 publications below

Product Information

55093-1-AP targets Frizzled 8 in WB, IHC, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Cited Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: Peptide
• Full Name: frizzled homolog 8 (Drosophila)
• Calculated Molecular Weight: 73 kDa
• Observed Molecular Weight: 70-73 kDa
• GenBank Accession Number: NM_031866
• Gene Symbol: Frizzled 8
• Gene ID (NCBI): 8325
• RRID: AB_2881267
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: 0.1M NaHCO3, 0.1M glycine, 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

Species	Application	Title
human	WB	Theranostics; Alkaline Phosphatase Controls Lineage Switching of Mesenchymal Stem Cells by Regulating the LRP6/GSK3β Complex in Hypophosphatasia.; Authors - Wenjia Liu