Frizzled 7 Polyclonal antibody

• Featured Product
• KD/KO Validated

Frizzled 7 Polyclonal Antibody for WB, IP, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IP, IF, RIP, IHC, CoIP, ELISA

Conjugate

Unconjugated

Publications

12

Reviews (2)

Cat no : 16974-1-AP

Synonyms

Frizzled 7, Fz 7, FZD7, FzE3, hFz7

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Validation Data Gallery

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  WB analysis of mouse skeletal muscle using 16974-1-AP

  mouse skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 16974-1-AP (Frizzled 7 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse heart using 16974-1-AP

  mouse heart tissue were subjected to SDS PAGE followed by western blot with 16974-1-AP (Frizzled 7 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse kidney using 16974-1-AP

  mouse kidney tissue were subjected to SDS PAGE followed by western blot with 16974-1-AP (Frizzled 7 antibody at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  IP experiment of mouse skeletal muscle using 16974-1-AP

  IP result of anti-Frizzled 7 (IP:16974-1-AP, 4ug; Detection:16974-1-AP 1:600) with mouse skeletal muscle tissue lysate 2200ug.

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  IHC staining of human small intestine using 16974-1-AP

  Immunohistochemical analysis of paraffin-embedded human small intestine tissue slide using 16974-1-AP (Frizzled 7 antibody) at dilution of 1:800 (under 10x lens).

• 

  IHC staining of human small intestine using 16974-1-AP

  Immunohistochemical analysis of paraffin-embedded human small intestine tissue slide using 16974-1-AP (Frizzled 7 antibody) at dilution of 1:800 (under 40x lens).

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Tested Applications

• Positive WB detected in: mouse skeletal muscle tissue, mouse heart tissue, mouse kidney tissue
• Positive IP detected in: mouse skeletal muscle tissue
• Positive IHC detected in: human small intestine tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:400-1:1600

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 2 publications below
• WB: See 10 publications below
• IHC: See 1 publications below
• IF: See 1 publications below
• CoIP: See 1 publications below
• RIP: See 1 publications below

Product Information

16974-1-AP targets Frizzled 7 in WB, IP, IF, RIP, IHC, CoIP, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: Frizzled 7 fusion protein Ag9446
• Full Name: frizzled homolog 7 (Drosophila)
• Calculated Molecular Weight: 574 aa, 64 kDa
• Observed Molecular Weight: 64 kDa
• GenBank Accession Number: BC015915
• Gene Symbol: Frizzled 7
• Gene ID (NCBI): 8324
• RRID: AB_2294535
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Frizzled class receptor 7 (Frizzled 7, also known as FZD7) is a member of the superfamily of G protein-coupled receptor proteins that act as receptors in the Wnt signaling pathway. Frizzled 7 plays a role in development, stem cell renewal, and differentiation and has been implicated in the progression of various cancers.

What is the molecular weight of Frizzled 7? Is Frizzled 7 post-translationally modified?

The molecular size of Frizzled 7 is ~63 kDa, which represents the mature N-glycosylated species (PMID: 25274627). Frizzled 7 can form homodimers and heterodimers with other Frizzled family members (PMID: 14688793).

What is the subcellular localization of Frizzled 7?

Frizzled 7 is a seven-pass transmembrane protein that is present at the plasma membrane. In endothelial cells, it predominantly locates to cell-cell junctions (PMID: 24866384). Frizzled 7 has a cleavage site for calpain-1 protease in its C-terminus that regulates its activity and plasma membrane turnover (PMID: 17716656).

What is the tissue expression pattern of Frizzled 7?

Frizzled 7 is expressed at high levels in adult skeletal muscles and in fetal kidneys, but is also present in fetal lung, adult heart, brain, and placenta (PMID: 9813155). Frizzled 7 expression is increased in various cancer types, including melanoma, hepatocellular carcinoma, lung, esophageal, gastric, and colon cancers.

Publications

Species	Application	Title
human,mouse	WB,IHC	Adv Mater; m6 A Reader YTHDF1-Targeting Engineered Small Extracellular Vesicles for Gastric Cancer Therapy via Epigenetic and Immune Regulation; Authors - Qing You
human	CoIP	Cell Death Differ; Cripto-1 contributes to stemness in hepatocellular carcinoma by stabilizing Dishevelled-3 and activating Wnt/β-catenin pathway.; Authors - Regina Cheuk-Lam Lo
human	WB	Mol Syst Biol; Systematic protein-protein interaction mapping for clinically relevant human GPCRs.; Authors - Kate Sokolina
human	WB	Cancer Res; YTHDF1 promotes gastric carcinogenesis by controlling translation of FZD7.; Authors - Jingnan Pi
mouse	WB,IF,RIP	Arch Med Res; RNA-binding Protein QKI Inhibits Osteogenic Differentiation Via Suppressing Wnt Pathway; Authors - Zhao Yan
human	WB	Front Microbiol; Expression and (Lacking) Internalization of the Cell Surface Receptors of Clostridioides difficile Toxin B.; Authors - Dennis Schöttelndreier

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Susanne (Verified Customer) (04-17-2023)

• Applications: Immunofluorescence
• Primary Antibody Dilution: 1:500
• Cell Tissue Type: Fibroblasts

Frizzled 7 Antibody Immunofluorescence validation (1:500 dilution) in Fibroblasts (Cat no:16974-1-AP)

FH

Louisiane (Verified Customer) (08-11-2022)

Tissues (small intestine sections) were incubated with primary antibody over the weekend at 4C and with secondary antibody overnight at 4C. The signal looks good

• Applications: Immunofluorescence
• Primary Antibody Dilution: 1 to 100