Frizzled 10 Polyclonal antibody

Frizzled 10 Polyclonal Antibody for WB, IHC, IF-P, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (3)

Applications

WB, IHC, IF-P, ELISA

Conjugate

Unconjugated

Publications

7

Reviews (2)

Cat no : 18175-1-AP

Synonyms

FZD10, FzE7, Fz-10, FZ 10, Frizzled-10

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Validation Data Gallery

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  WB analysis of mouse kidney using 18175-1-AP

  mouse kidney tissue were subjected to SDS PAGE followed by western blot with 18175-1-AP (Frizzled 10 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 18175-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 18175-1-AP (Frizzled 10 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 18175-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 18175-1-AP (Frizzled 10 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human kidney using 18175-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney using 18175-1-AP (Frizzled 10 antibody) at dilution of 1:100 (under 10x lens).

• 

  IHC staining of human kidney using 18175-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney using 18175-1-AP (Frizzled 10 antibody) at dilution of 1:100 (under 40x lens).

• 

  IF Staining of human kidney using 18175-1-AP

  Immunofluorescent analysis of (4% PFA) fixed human kidney tissue using Frizzled 10 antibody (18175-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

• 

  IF Staining of human kidney using 18175-1-AP

  Immunofluorescent analysis of (4% PFA) fixed human kidney tissue using Frizzled 10 antibody (18175-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

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Tested Applications

• Positive WB detected in: mouse kidney tissue, HeLa cells
• Positive IHC detected in: human kidney tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF-P detected in: human kidney tissue

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Immunohistochemistry (IHC): IHC : 1:20-1:200
• Immunofluorescence (IF)-P: IF-P : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 5 publications below
• IHC: See 2 publications below
• IF: See 1 publications below
• FC: See 1 publications below

Product Information

18175-1-AP targets Frizzled 10 in WB, IHC, IF-P, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse, chicken, zebrafish, goat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: Frizzled 10 fusion protein Ag12867
• Full Name: frizzled homolog 10 (Drosophila)
• Calculated Molecular Weight: 581 aa, 65 kDa
• Observed Molecular Weight: 65 kDa
• GenBank Accession Number: BC074998
• Gene Symbol: Frizzled 10
• Gene ID (NCBI): 11211
• RRID: AB_2108942
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

FZD10 (Frizzled homolog 10) is a member of the frizzled gene family, and considered as a seven-transmembrane Wnt-signaling receptor. FZD10 is expressed at high levels on the cell surface of almost all synovial sarcoma tissues but absent in most normal organs except for placenta. Up-regulation of FZD10 mRNA in several types of human cells might lead to carcinogenesis through activation of the beta-catenin-TCF signaling pathway with some class of WNTs.

Publications

Species	Application	Title
mouse	WB,FC	Nat Commun; Progenitor translatome changes coordinated by Tsc1 increase perception of Wnt signals to end nephrogenesis.; Authors - Alison E Jarmas
chicken	IHC,IF	Development; Coupling of apical-basal polarity and PCP to interpret the Wnt signaling gradient and orient feather branch.; Authors - Jianqiong Lin
zebrafish	WB	Development; Wnt/β-catenin signaling directly regulates Foxj1 expression and ciliogenesis in zebrafish Kupffer's vesicle.; Authors - Alissa Caron
human	WB	Oncotarget; Non coding RNA analysis in fibrolamellar hepatocellular carcinoma.; Authors - Benjamin A Farber
human	WB	Int J Oncol; Notch1 promotes hepatitis B virus X protein-induced hepatocarcinogenesis via Wnt/β-catenin pathway.; Authors - Qian Sun
goat	IHC	Cells; Integrative Analysis of Methylome and Transcriptome Reveals the Regulatory Mechanisms of Hair Follicle Morphogenesis in Cashmere Goat.; Authors - Shanhe Wang

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Susanne (Verified Customer) (04-17-2023)

staining was not so nice like Frizzled 5, 7 or 9 from Proteintech

• Applications: Immunofluorescence
• Primary Antibody Dilution: 1:50
• Cell Tissue Type: Fibroblasts

Frizzled 10 Antibody Immunofluorescence validation (1:50 dilution) in Fibroblasts (Cat no:18175-1-AP)

FH

Sylvain (Verified Customer) (12-11-2018)

Perfect signal!

• Applications: Immunohistochemistry,
• Primary Antibody Dilution: 1:100
• Cell Tissue Type: human colon cancer