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  • KD/KO Validated

FYN Monoclonal antibody

FYN Monoclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human, Mouse, Rat and More (3)

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

CloneNo.

1G4B1

Cat no : 66606-1-Ig

Synonyms

FYN, p59 Fyn, Proto oncogene c Fyn, Proto oncogene Syn, SLK, Src like kinase, SYN, Tyrosine protein kinase Fyn



Tested Applications

Positive WB detected inHEK-293 cells, Jurkat cells, Ramos cells, HeLa cells, MCF-7 cells, HSC-T6 cells, NIH/3T3 cells
Positive IHC detected inhuman tonsillitis tissue, human breast cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inNeuro-2a cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:30000
Immunohistochemistry (IHC)IHC : 1:2000-1:5000
Immunofluorescence (IF)/ICCIF/ICC : 1:400-1:1600
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

66606-1-Ig targets FYN in WB, IHC, IF/ICC, ELISA applications and shows reactivity with Human, Mouse, Rat samples.

Tested Reactivity Human, Mouse, Rat
Cited Reactivityhuman, mouse, rat
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen FYN fusion protein Ag11127
Full Name FYN oncogene related to SRC, FGR, YES
Calculated Molecular Weight537aa,61 kDa; 482aa,55 kDa
Observed Molecular Weight 60 kDa
GenBank Accession NumberBC032496
Gene Symbol FYN
Gene ID (NCBI) 2534
RRIDAB_2881966
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

FYN, also named as p59-Fyn and SLK, belongs to the protein kinase superfamily, Tyr protein kinase family and SRC subfamily. FYN is implicated in the control of cell growth. It plays a role in the regulation of intracellular calcium levels, with isoform 2 showing the greater ability to mobilize cytoplasmic calcium in comparison to isoform 1. FYN is required in brain development and mature brain function with important roles in the regulation of axon growth, axon guidance, and neurite extension. FYN blocks axon outgrowth and attraction induced by NTN1 by phosphorylating its receptor DDC.

Protocols

Product Specific Protocols
WB protocol for FYN antibody 66606-1-IgDownload protocol
IHC protocol for FYN antibody 66606-1-IgDownload protocol
IF protocol for FYN antibody 66606-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseWB

EBioMedicine

Obesity induces preadipocyte CD36 expression promoting inflammation via the disruption of lysosomal calcium homeostasis and lysosome function.

Authors - Xiaoxiao Luo
human,mouseIHC

EBioMedicine

Biallelic mutations in LAMA5 disrupts a skeletal noncanonical focal adhesion pathway and produces a distinct bent bone dysplasia.

Authors - Maya Barad
humanWB

Biochem Pharmacol

PIKE-A promotes glioblastoma growth by driving PPP flux through increasing G6PD expression mediated by phosphorylation of STAT3.

Authors - Mingming Sun
humanIHC

Front Oncol

A four oxidative stress gene prognostic model and integrated immunity-analysis in pancreatic adenocarcinoma

Authors - Hao Wang
humanWB

Exp Cell Res

ISG15 promotes esophageal squamous cell carcinoma tumorigenesis via c-MET/Fyn/β-catenin signaling pathway.

Authors - Hongyu Yuan
humanWB

Exp Cell Res

Src-mediated Tyr353 phosphorylation of IP3R1 promotes its stability and causes apoptosis in palmitic acid-treated hepatocytes.

Authors - Ting Yu

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.


FH

X (Verified Customer) (07-11-2022)

A reliable antibody in WB.

  • Applications: Western Blot
  • Primary Antibody Dilution: 1:2000
  • Cell Tissue Type: mouse brain lysate
FYN Antibody Western Blot validation (1:2000 dilution) in mouse brain lysate (Cat no:66606-1-Ig)