FYN Polyclonal antibody

• 

  WB analysis using 17237-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 17237-1-AP (FYN antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

• 

  IHC staining of rat testis using 17237-1-AP

  Immunohistochemical analysis of paraffin-embedded rat testis tissue slide using 17237-1-AP (FYN antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of rat testis using 17237-1-AP

  Immunohistochemical analysis of paraffin-embedded rat testis tissue slide using 17237-1-AP (FYN antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse spleen using 17237-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse spleen tissue slide using 17237-1-AP (FYN antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse spleen using 17237-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse spleen tissue slide using 17237-1-AP (FYN antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

FYN Polyclonal Antibody for WB, IHC, ELISA

Cat No. 17237-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Synonyms

Proto-oncogene c-Fyn, Proto oncogene Syn, Proto oncogene c Fyn, p59-Fyn, p59 Fyn

---

Tested Applications

• Positive WB detected in: A431 cells, HEK-293 cells, HeLa cells, K-562 cells
• Positive IHC detected in: rat testis tissue, mouse spleen tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:8000
• Immunohistochemistry (IHC): IHC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

17237-1-AP targets FYN in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: FYN fusion protein Ag11080
• Full Name: FYN oncogene related to SRC, FGR, YES
• Calculated Molecular Weight: 537aa,61 kDa; 482aa,55 kDa
• Observed Molecular Weight: 60 kDa
• GenBank Accession Number: BC032496
• Gene Symbol: FYN
• Gene ID (NCBI): 2534
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: P06241
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

FYN, also named p59-Fyn and SLK, belongs to the protein kinase superfamily, Tyr protein kinase family, and SRC subfamily. FYN is implicated in the control of cell growth. It plays a role in the regulation of intracellular calcium levels, with isoform 2 showing a greater ability to mobilize cytoplasmic calcium in comparison to isoform 1. FYN is required in brain development and mature brain function with important roles in the regulation of axon growth, axon guidance, and neurite extension. FYN blocks axon outgrowth and attraction induced by NTN1 by phosphorylating its receptor DDC.