FXYD2 Recombinant antibody

FXYD2 Uni-rAb^TM Recombinant Antibody for WB, FC (Intra), ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, FC (Intra), ELISA

Conjugate

Unconjugated

CloneNo.

240649C2

Cat no : 83860-1-RR

Synonyms

Sodium/potassium-transporting ATPase subunit gamma, FXYD domain-containing ion transport regulator 2, FXYD 2, ATP1G1, ATP1C

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Validation Data Gallery

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  WB analysis of human kidney using 83860-1-RR

  human kidney tissue were subjected to SDS PAGE followed by western blot with 83860-1-RR (FXYD2 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

• 

  FC experiment of HepG2 using 83860-1-RR

  1x10^6 HepG2 cells were intracellularly stained with 0.25 ug FXYD2 Recombinant antibody (83860-1-RR, Clone:240649C2) and APC-Conjugated Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

• 

  Affinity and Kinetic Characterization of 83860-1-RR

  Biolayer interferometry (BLl) kinetic assays of 83860-1-RR against Human FXYD2 were performed. The affinity constant is below 1 pM.

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Tested Applications

• Positive WB detected in: human kidney tissue
• Positive FC (Intra) detected in: HepG2 cells

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:6000
• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of 83860-1-RR is FXYD2 Fusion Protein expressed in E. coli.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: FXYD2 fusion protein Ag34030
• Full Name: FXYD domain containing ion transport regulator 2
• Calculated Molecular Weight: 7 kDa
• Observed Molecular Weight: 7-10 kDa
• GenBank Accession Number: BC013289
• Gene Symbol: FXYD2
• Gene ID (NCBI): 486
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purfication
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

FXYD2 (FXYD domain-containing ion transport regulator 2), also known as the gamma-subunit of the NaK-ATPase, belongs to the FXYD family which has been proposed to be the regulators of Na, K-ATPase function by lowering affinities of the system for potassium and sodium. The expression of FXYD2 is most abundant in kidney, while it is also detected in several other tissues like placenta, pancreas, and dorsal root ganglia (DRGs). Three splice variants of FXYD2 have been reported in mouse kidney, namely FXYD2 γa, -γb,and -γc. FXYD2 γa has been identified as a pancreatic beta cell-specific biomarker. This antibody can recognize all three isoforms of FXYD2.