FUT9 Monoclonal antibody, PBS Only

• Featured Product
• KD/KO Validated

FUT9 Monoclonal Antibody for WB, IHC, IF-P, Indirect ELISA

Host / Isotype

Mouse / IgG2a

Reactivity

human, pig, mouse, rat

Applications

WB, IHC, IF-P, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

5D4G6

Cat no : 60230-1-PBS

Synonyms

Alpha (1,3) fucosyltransferase, Fuc TIX, Fucosyltransferase 9, Fucosyltransferase IX, FucT IX, FUT9

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Validation Data Gallery

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  WB analysis of pig brain using 60230-1-Ig (same clone as 60230-1-PBS)

  pig brain tissue were subjected to SDS PAGE followed by western blot with 60230-1-Ig (FUT9 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60230-1-PBS in a different storage buffer formulation.

• 

  WB analysis of rat brain using 60230-1-Ig (same clone as 60230-1-PBS)

  rat brain tissue were subjected to SDS PAGE followed by western blot with 60230-1-Ig (FUT9 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60230-1-PBS in a different storage buffer formulation.

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  WB analysis of mouse brain using 60230-1-Ig (same clone as 60230-1-PBS)

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 60230-1-Ig (FUT9 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60230-1-PBS in a different storage buffer formulation.

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  WB analysis of fetal human brain using 60230-1-Ig (same clone as 60230-1-PBS)

  fetal human brain tissue were subjected to SDS PAGE followed by western blot with 60230-1-Ig (FUT9 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60230-1-PBS in a different storage buffer formulation.

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  IHC staining of human cervical cancer using 60230-1-Ig (same clone as 60230-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 60230-1-Ig (FUT9 Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60230-1-PBS in a different storage buffer formulation.

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  IHC staining of human cervical cancer using 60230-1-Ig (same clone as 60230-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 60230-1-Ig (FUT9 Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60230-1-PBS in a different storage buffer formulation.

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  IHC staining of human breast cancer using 60230-1-Ig (same clone as 60230-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 60230-1-Ig (FUT9 Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60230-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human breast cancer using 60230-1-Ig (same clone as 60230-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 60230-1-Ig (FUT9 Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60230-1-PBS in a different storage buffer formulation.

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  IHC staining of human stomach using 60230-1-Ig (same clone as 60230-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human stomach using 60230-1-Ig(FUT9 antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 60230-1-PBS in a different storage buffer formulation.

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  IHC staining of human stomach using 60230-1-Ig (same clone as 60230-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human stomach using 60230-1-Ig(FUT9 antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 60230-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human stomach using 60230-1-Ig (same clone as 60230-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human stomach slide using 60230-1-Ig (FUT9 Antibody) at dilution of 1:50. This data was developed using the same antibody clone with 60230-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human stomach using 60230-1-Ig (same clone as 60230-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human stomach slide using 60230-1-Ig (FUT9 Antibody) at dilution of 1:50. This data was developed using the same antibody clone with 60230-1-PBS in a different storage buffer formulation.

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  IF Staining of human breast cancer using 60230-1-Ig (same clone as 60230-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed human breast cancer tissue using FUT9 antibody (60230-1-Ig, Clone: 5D4G6 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 60230-1-PBS in a different storage buffer formulation.

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Product Information

60230-1-PBS targets FUT9 in WB, IHC, IF-P, Indirect ELISA applications and shows reactivity with human, pig, mouse, rat samples.

• Tested Reactivity: human, pig, mouse, rat
• Host / Isotype: Mouse / IgG2a
• Class: Monoclonal
• Type: Antibody
• Immunogen: FUT9 fusion protein Ag8298
• Full Name: fucosyltransferase 9 (alpha (1,3) fucosyltransferase)
• Calculated Molecular Weight: 359 aa, 42 kDa
• Observed Molecular Weight: 44-46 kDa
• GenBank Accession Number: BC036101
• Gene Symbol: FUT9
• Gene ID (NCBI): 10690
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.

Background Information

FUT9 is the main enzyme responsible for the synthesis of Lewis X (Lex) and catalyzes the last step in the biosynthesis of Lewis X antigen by addition of a fucose residue to precursor glycan structures. FUT9 is expressed in stomach, kidney, brain, and in leukocytes.