FUT8 Polyclonal antibody

FUT8 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 29430-1-AP

Synonyms

Alpha (1,6) fucosyltransferase, alpha1 6FucT, Fucosyltransferase 8, FUT8

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Validation Data Gallery

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  WB analysis using 29430-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 29430-1-AP (FUT8 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IHC staining of human lung cancer using 29430-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 29430-1-AP (FUT8 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human breast cancer using 29430-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 29430-1-AP (FUT8 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

• Positive WB detected in: MDA-MB-231 cells, NCI-H1299 cells
• Positive IHC detected in: human lung cancer tissue, human breast cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

29430-1-AP targets FUT8 in WB, IHC, ELISA applications and shows reactivity with Human samples.

• Tested Reactivity: Human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: FUT8 fusion protein Ag30784
• Full Name: fucosyltransferase 8 (alpha (1,6) fucosyltransferase)
• Calculated Molecular Weight: 66 kDa
• Observed Molecular Weight: 60-66 kDa
• GenBank Accession Number: BC025385
• Gene Symbol: FUT8
• Gene ID (NCBI): 2530
• RRID: AB_2918305
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.