FUT11 Polyclonal antibody

FUT11 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Publications

4

Cat no : 17175-1-AP

Synonyms

Fuc TXI, Fucosyltransferase 11, Fucosyltransferase XI, FucT XI, FUT11

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Validation Data Gallery

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  WB analysis of mouse lung using 17175-1-AP

  mouse lung tissue were subjected to SDS PAGE followed by western blot with 17175-1-AP (FUT11 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse testis using 17175-1-AP

  mouse testis tissue were subjected to SDS PAGE followed by western blot with 17175-1-AP (FUT11 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human testis using 17175-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis using 17175-1-AP (FUT11 antibody) at dilution of 1:100 (under 10x lens).

• 

  IHC staining of human testis using 17175-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis using 17175-1-AP (FUT11 antibody) at dilution of 1:100 (under 40x lens).

• 

  IHC staining of human brain using 17175-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain using 17175-1-AP (FUT11 antibody) at dilution of 1:100 (under 10x lens).

• 

  IHC staining of human brain using 17175-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain using 17175-1-AP (FUT11 antibody) at dilution of 1:100 (under 40x lens).

• 

  IHC staining of human spleen using 17175-1-AP

  Immunohistochemical analysis of paraffin-embedded human spleen using 17175-1-AP (FUT11 antibody) at dilution of 1:100 (under 10x lens).

• 

  IHC staining of human spleen using 17175-1-AP

  Immunohistochemical analysis of paraffin-embedded human spleen using 17175-1-AP (FUT11 antibody) at dilution of 1:100 (under 40x lens).

• 

  IHC staining of human lung using 17175-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung using 17175-1-AP (FUT11 antibody) at dilution of 1:100 (under 10x lens).

• 

  IHC staining of human lung using 17175-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung using 17175-1-AP (FUT11 antibody) at dilution of 1:100 (under 40x lens).

• 

  IHC staining of human ovary using 17175-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary using 17175-1-AP (FUT11 antibody) at dilution of 1:100 (under 10x lens).

• 

  IHC staining of human ovary using 17175-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary using 17175-1-AP (FUT11 antibody) at dilution of 1:100 (under 40x lens).

• 

  IHC staining of human kidney using 17175-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney using 17175-1-AP (FUT11 antibody) at dilution of 1:100 (under 10x lens).

• 

  IHC staining of human kidney using 17175-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney using 17175-1-AP (FUT11 antibody) at dilution of 1:100 (under 40x lens).

• 

  IHC staining of human placenta using 17175-1-AP

  Immunohistochemical analysis of paraffin-embedded human placenta using 17175-1-AP (FUT11 antibody) at dilution of 1:100 (under 10x lens).

• 

  IHC staining of human placenta using 17175-1-AP

  Immunohistochemical analysis of paraffin-embedded human placenta using 17175-1-AP (FUT11 antibody) at dilution of 1:100 (under 40x lens).

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Tested Applications

• Positive WB detected in: mouse lung tissue, mouse testis tissue
• Positive IHC detected in: human testis tissue, human brain tissue, human kidney tissue, human lung tissue, human ovary tissue, human placenta tissue, human spleen tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:3000
• Immunohistochemistry (IHC): IHC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 3 publications below
• IHC: See 2 publications below

Product Information

17175-1-AP targets FUT11 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: FUT11 fusion protein Ag10815
• Full Name: fucosyltransferase 11 (alpha (1,3) fucosyltransferase)
• Calculated Molecular Weight: 492 aa, 56 kDa
• Observed Molecular Weight: 56 kDa
• GenBank Accession Number: BC036037
• Gene Symbol: FUT11
• Gene ID (NCBI): 170384
• RRID: AB_2231850
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

Species	Application	Title
human	WB	Int Immunopharmacol; Dry and wet experiments reveal the significant role of FUT11 in clear cell renal cell carcinoma; Authors - Pu Zhang
human	WB	Cell Biol Int; FUT11 is a target gene of HIF1α that promotes the progression of hepatocellular carcinoma.; Authors - Wanyuan Ruan
human	WB,IHC	Heliyon; Knockdown of FUT11 inhibits the progression of gastric cancer via the PI3K/AKT pathway; Authors - Wenpeng Cao
human	IHC	Discov Oncol; FUT11 expression in gastric cancer: its prognostic significance and role in immune regulation; Authors - Yanqing Huang