FUT10 Polyclonal antibody

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  WB analysis of HeLa using 18660-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 18660-1-AP (FUT10 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  IHC staining of human testis using 18660-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis using 18660-1-AP (FUT10 antibody) at dilution of 1:100 (under 10x lens).

• 

  IHC staining of human testis using 18660-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis using 18660-1-AP (FUT10 antibody) at dilution of 1:100 (under 40x lens).

FUT10 Polyclonal Antibody for WB, IHC, ELISA

Cat No. 18660-1-AP

Publications(3)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF, ELISA

Synonyms

Fuc TX, Fucosyltransferase 10, Fucosyltransferase X, FucT X, FUT10

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Tested Applications

• Positive WB detected in: HeLa cells
• Positive IHC detected in: human testis tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:3000
• Immunohistochemistry (IHC): IHC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 1 publications below
• IF: See 2 publications below

Product Information

18660-1-AP targets FUT10 in WB, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: FUT10 fusion protein Ag13415
• Full Name: fucosyltransferase 10 (alpha (1,3) fucosyltransferase)
• Calculated Molecular Weight: 521 aa, 61 kDa
• Observed Molecular Weight: 41-48 kDa
• GenBank Accession Number: BC063462
• Gene Symbol: FUT10
• Gene ID (NCBI): 84750
• RRID: AB_10641997
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q6P4F1
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

Species	Application	Title
mouse	IF	Elife; The role of the NMD factor UPF3B in olfactory sensory neurons.; Authors - Kun Tan
human	WB	Arthritis Res Ther; Elevation of α-1,3 fucosylation promotes the binding ability of TNFR1 to TNF-α and contributes to osteoarthritic cartilage destruction and apoptosis.; Authors - Hanjie Yu
human	IF	Nat Chem Biol; FUT10 and FUT11 are protein O-fucosyltransferases that modify protein EMI domains; Authors - Huilin Hao