FURIN Polyclonal antibody

• Featured Product
• KD/KO Validated

FURIN Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human and More (3)

Applications

WB, IHC, IF/ICC, IP, ELISA

Conjugate

Unconjugated

Publications

23

Reviews (4)

Cat no : 18413-1-AP

Synonyms

PCSK3, Paired basic amino acid residue-cleaving enzyme, PACE, FUR, EC:3.4.21.75

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Validation Data Gallery

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  WB analysis of HepG2 using 18413-1-AP

  WB result of FURIN antibody (18413-1-AP; 1:2000; incubated at room temperature for 1.5 hours) with sh-Control and sh-FURIN transfected HepG2 cells.

• 

  WB analysis of COLO 320 using 18413-1-AP

  COLO 320 cells were subjected to SDS PAGE followed by western blot with 18413-1-AP (FURIN Antibody) at dilution of 1:300 incubated at 4 degree celsius over night.

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  WB analysis of HeLa using 18413-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 18413-1-AP (FURIN Antibody) at dilution of 1:300 incubated at 4 degree celsius over night.

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  IP experiment of HeLa using 18413-1-AP

  IP result of anti-FURIN (IP:18413-1-AP, 4ug; Detection:18413-1-AP 1:4000) with HeLa cells lysate 2000 ug.

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  IHC staining of human placenta using 18413-1-AP

  Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 18413-1-AP (FURIN Antibody) at dilution of 1:200 (under 10x lens).

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  IHC staining of human placenta using 18413-1-AP

  Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 18413-1-AP (FURIN Antibody) at dilution of 1:200 (under 40x lens).

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  IF Staining of HeLa using 18413-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using FURIN antibody (18413-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2).

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Tested Applications

• Positive WB detected in: HepG2 cells, COLO 320 cells, HeLa cells
• Positive IP detected in: HeLa cells
• Positive IHC detected in: human placenta tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: HeLa cells

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:4000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:20-1:200
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 5 publications below
• WB: See 19 publications below
• IHC: See 3 publications below
• IF: See 2 publications below

Product Information

18413-1-AP targets FURIN in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Cited Reactivity: human, mouse, rat, chicken
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: FURIN fusion protein Ag13261
• Full Name: furin (paired basic amino acid cleaving enzyme)
• Calculated Molecular Weight: 87 kDa
• Observed Molecular Weight: 90-110 kDa
• GenBank Accession Number: BC012181
• Gene Symbol: FURIN
• Gene ID (NCBI): 5045
• RRID: AB_2878542
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

FURIN, also named FUR, PACE, PCSK3, SPC1, and Kex2p, is likely to represent the ubiquitous endoprotease activity within constitutive secretory pathways and is capable of cleavage at the RX(K/R)R consensus motif. Furin is synthesized as an inactive zymogen that may minimize the occurrence of premature enzymatic activity that would lead to alternative protein activation or degradation. The molecular weight of mature furin is 75-90 kDa and that of pre-pro furin is 95 kDa. With glycosylation and other post-translational modifications, the molecular weight of pre-pro furin will be migrated to 100-110 kDa. (PMID: 1644796, 1885622, 31336100, 35322530).

Publications

Species	Application	Title
human	WB	Adv Sci (Weinh); Furin Egress from the TGN is Regulated by Membrane-Associated RING-CH Finger (MARCHF) Proteins and Ubiquitin-Specific Protease 32 (USP32) via Nondegradable K33-Polyubiquitination; Authors - Wenqiang Su ; KD Validated
human	IHC	Cell Rep; Deep spatial proteomics reveals region-specific features of severe COVID-19-related pulmonary injury; Authors - Yiheng Mao
human	WB	Oncogene; Lipoic acid-induced oxidative stress abrogates IGF-1R maturation by inhibiting the CREB/furin axis in breast cancer cell lines.; Authors - Diana Farhat
human	WB	PLoS Pathog; Antibody-mediated spike activation promotes cell-cell transmission of SARS-CoV-2; Authors - Shi Yu ; KD Validated
human	WB	Oncogenesis; Methyl-CpG-binding protein 2 drives the Furin/TGF-β1/Smad axis to promote epithelial-mesenchymal transition in pancreatic cancer cells.; Authors - Huizhi Wang
human	WB	Br J Cancer; Lipoic acid decreases breast cancer cell proliferation by inhibiting IGF-1R via furin downregulation.; Authors - Diana Farhat ; KD Validated

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Wojciech (Verified Customer) (05-31-2024)

WB at 1:1000. Two (80 and 104 kDa) bands detected.

• Applications: Western Blot
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: T-ALL cell lines

FH

Wojciech (Verified Customer) (05-21-2024)

Used in WB and got 2 bands at 81 and 104 kDa at 1:1000 dilution.

• Applications: Western Blot
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: T-ALL cell lines

FH

Boyan (Verified Customer) (03-11-2019)

For WB, the expected band was strong; for IF, PFA fixation, it could label the cell surface-like localisation and the Golgi localisation of Furin.

• Applications: Western Blot, Immunofluorescence,
• Cell Tissue Type: MEF

FH

Alex (Verified Customer) (10-23-2018)

Cells were fixed with 4% PFA, permeabilized with saponin, and stained with anti-furin.

• Applications: Immunofluorescence,
• Primary Antibody Dilution: 1:200
• Cell Tissue Type: 293T, HeLa