FUNDC1 Polyclonal antibody

• Featured Product
• KD/KO Validated

FUNDC1 Polyclonal Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human, mouse, rat and More (2)

Applications

WB, ELISA

Conjugate

Unconjugated

Publications

4

Cat no : 28519-1-AP

Synonyms

FUN14 domain containing 1, FUNDC1

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Validation Data Gallery

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  WB analysis using 28519-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 28519-1-AP (FUNDC1 antibody) at dilution of 1:40000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 28519-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 28519-1-AP (FUNDC1 antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.

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Tested Applications

• Positive WB detected in: RAW 264.7 cells, RAW 264, .7 cells, mouse brain tissue, mouse liver tissue, rat brain tissue, rat liver tissue, C6 cells

Recommended dilution

• Western Blot (WB): WB : 1:5000-1:20000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 1 publications below
• WB: See 4 publications below

Product Information

28519-1-AP targets FUNDC1 in WB, ELISA applications and shows reactivity with Human, mouse, rat samples.

• Tested Reactivity: Human, mouse, rat
• Cited Reactivity: human, mouse, goat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: FUNDC1 fusion protein Ag29358
• Full Name: FUN14 domain containing 1
• Calculated Molecular Weight: 155 aa, 17 kDa
• Observed Molecular Weight: 17 kDa
• GenBank Accession Number: BC042813
• Gene Symbol: FUNDC1
• Gene ID (NCBI): 139341
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

FUNDC1 is a novel mitochondrial-associated membrane (MAM) protein, enriched at the MAM by interacting with the ER resident protein CANX (calnexin) under hypoxia. As mitophagy proceeds, it dissociates from CANX and preferably recruits DNM1L/DRP1 to drive mitochondrial fission in response to hypoxic stress.

Publications

Species	Application	Title
mouse	WB	Clin Sci (Lond); Loss of FoxO1 activates an alternate mechanism of mitochondrial quality control for healthy adipose browning; Authors - Limin Shi ; KD Validated
goat	WB	FASEB J; Zearalenone modulates the function of goat endometrial cells via the mitochondrial quality control system; Authors - Guomin Zhang
mouse	WB	Mech Ageing Dev; Deletion of Stimulator of Interferons Genes Aggravated Cardiac Dysfunction in Physiological Aged Mice; Authors - Diansa Gao
human	WB	Redox Biol; VDR regulates mitochondrial function as a protective mechanism against renal tubular cell injury in diabetic rats; Authors - Hong Chen