FTSJ1 Polyclonal antibody

FTSJ1 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

Publications

1

Cat no : 11620-1-AP

Synonyms

MRX44, JM23, FtsJ homolog 1 (E. coli), EC:2.1.1.205, CDLIV

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Validation Data Gallery

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  WB analysis of HeLa using 11620-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 11620-1-AP (FTSJ1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  IHC staining of human liver using 11620-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 11620-1-AP (FTSJ1 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human liver using 11620-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 11620-1-AP (FTSJ1 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse brain using 11620-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 11620-1-AP (FTSJ1 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse brain using 11620-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 11620-1-AP (FTSJ1 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of A431 using 11620-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed A431 cells using FTSJ1 antibody (11620-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

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Tested Applications

• Positive WB detected in: HeLa cells, HepG2 cells, U-87 MG cells
• Positive IHC detected in: human liver tissue, mouse brain tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: A431 cells

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:4000
• Immunohistochemistry (IHC): IHC : 1:500-1:2000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 1 publications below

Product Information

11620-1-AP targets FTSJ1 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: FTSJ1 fusion protein Ag2199
• Full Name: FtsJ homolog 1 (E. coli)
• Calculated Molecular Weight: 329 aa, 36 kDa
• Observed Molecular Weight: 36 kDa
• GenBank Accession Number: BC023584
• Gene Symbol: FTSJ1
• Gene ID (NCBI): 24140
• RRID: AB_2877785
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

Species	Application	Title
human	WB	bioRxiv; Dengue virus exploits the host tRNA epitranscriptome to promote viral replication; Authors - Cheryl Chan