FSTL1 Polyclonal antibody

• Featured Product
• KD/KO Validated

FSTL1 Polyclonal Antibody for WB, IHC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, rat and More (1)

Applications

WB, IHC, IF, IP, CoIP, ELISA

Conjugate

Unconjugated

Publications

15

Cat no : 20182-1-AP

Synonyms

FSTL 1, Follistatin-related protein 1, Follistatin-like protein 1, Follistatin related protein 1, Follistatin like protein 1

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Validation Data Gallery

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  WB analysis using 20182-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 20182-1-AP (FSTL1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 20182-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 20182-1-AP (FSTL1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 20182-1-AP

  WB result of FSTL1 antibody (20182-1-AP; 1:2000; incubated at room temperature for 1.5 hours) with sh-Control and sh-FSTL1 transfected HeLa cells.

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  WB analysis of human placenta using 20182-1-AP

  human placenta tissue were subjected to SDS PAGE followed by western blot with 20182-1-AP (FSTL1 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IP experiment of A2780 using 20182-1-AP

  IP result of anti-FSTL1 (IP:20182-1-AP, 4ug; Detection:20182-1-AP 1:2000) with A2780 cells lysate 960 ug.

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  IHC staining of human placenta using 20182-1-AP

  Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 20182-1-AP (FSTL1 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human placenta using 20182-1-AP

  Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 20182-1-AP (FSTL1 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human colon using 20182-1-AP

  Immunohistochemical analysis of paraffin-embedded human normal colon slide using 20182-1-AP (FSTL1 antibody) at dilution of 1:400 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human colon using 20182-1-AP

  Immunohistochemical analysis of paraffin-embedded human normal colon slide using 20182-1-AP (FSTL1 antibody) at dilution of 1:400 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human gliomas using 20182-1-AP

  Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 20182-1-AP (FSTL1 Antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human gliomas using 20182-1-AP

  Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 20182-1-AP (FSTL1 Antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human pancreas using 20182-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas tissue slide using 20182-1-AP (FSTL1 Antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human pancreas using 20182-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas tissue slide using 20182-1-AP (FSTL1 Antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of rat colon using 20182-1-AP

  Immunohistochemical analysis of paraffin-embedded rat colon tissue slide using 20182-1-AP (FSTL1 Antibody) at dilution of 1:200 (under 10x lens).

• 

  IHC staining of rat colon using 20182-1-AP

  Immunohistochemical analysis of paraffin-embedded rat colon tissue slide using 20182-1-AP (FSTL1 Antibody) at dilution of 1:200 (under 40x lens).

• 

  IHC staining of rat testis using 20182-1-AP

  Immunohistochemical analysis of paraffin-embedded rat testis tissue slide using 20182-1-AP (FSTL1 Antibody) at dilution of 1:200 (under 10x lens).

• 

  IHC staining of rat testis using 20182-1-AP

  Immunohistochemical analysis of paraffin-embedded rat testis tissue slide using 20182-1-AP (FSTL1 Antibody) at dilution of 1:200 (under 40x lens).

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Tested Applications

• Positive WB detected in: A2780 cells, HeLa cells, human placenta tissue, A431 cells, C6 cells
• Positive IP detected in: A2780 cells
• Positive IHC detected in: human colon tissue, human gliomas tissue, human pancreas tissue, human placenta tissue, rat colon tissue, rat testis tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:2000-1:10000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 4 publications below
• WB: See 11 publications below
• IHC: See 8 publications below
• IF: See 3 publications below
• IP: See 1 publications below
• CoIP: See 1 publications below

Product Information

20182-1-AP targets FSTL1 in WB, IHC, IF, IP, CoIP, ELISA applications and shows reactivity with human, rat samples.

• Tested Reactivity: human, rat
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: FSTL1 fusion protein Ag14057
• Full Name: follistatin-like 1
• Calculated Molecular Weight: 308 aa, 35 kDa
• Observed Molecular Weight: 35-40 kDa
• GenBank Accession Number: BC000055
• Gene Symbol: FSTL1
• Gene ID (NCBI): 11167
• RRID: AB_10666439
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Follistatin-like 1 (FSTL1), initially discovered as a TGF-β1-induced gene, encodes a 308 amino acid secreted 45-55 kDa glycoprotein with a follistatin domain and two non-functional calcium-binding motifs. FSTL1 has been reported to be involved in the fate determination and maturation of epithelial cells. Ablation of the FSTL1 gene in the mouse results in several structural developmental defects and neonatal lethality due to respiratory failure, demonstrating its functional importance. FSTL1 has been reported to exhibit both pro- and anti-inflammatory actions, with a specific anti-apoptotic and protective effect in cardiac or renal cell injury. Zwijsen et al. (1994) detected several isoforms of FSTL1 with molecular masses of 40 to 48 kD which differs from the 50- to 55-kD products detected by Tanaka et al. (1998) (OMIM).

Publications

Species	Application	Title
human	WB,IF	J Exp Med; EGF hijacks miR-198/FSTL1 wound-healing switch and steers a two-pronged pathway toward metastasis.; Authors - Gopinath M Sundaram
mouse	IHC	Bone Res; Single cell analysis reveals inhibition of angiogenesis attenuates the progression of heterotopic ossification in Mkx-/- mice.; Authors - Junxin Lin
human	WB	Cancer Res; Follistatin-like Protein 1 Inhibits Lung Cancer Metastasis by Preventing Proteolytic Activation of Osteopontin.; Authors - Jean Chiou ; KD Validated
human,mouse	WB,IHC,IP	Cell Death Dis; FSTL1 interacts with VIM and promotes colorectal cancer metastasis via activating the focal adhesion signalling pathway.; Authors - Chuansha Gu
mouse	IHC	Aging (Albany NY); Follistatin-like 1 deficiency impairs T cell development to promote lung metastasis of triple negative breast cancer.; Authors - Jie Ma ; KO Validated
human	IHC,CoIP	Am J Cancer Res; FSTL1 promotes growth and metastasis in gastric cancer by activating AKT related pathway and predicts poor survival.; Authors - Mengjie Wu ; KD Validated

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