FSHR Polyclonal antibody

• Featured Product
• KD/KO Validated

FSHR Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (6)

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

Publications

92

Reviews (1)

Cat no : 22665-1-AP

Synonyms

Follitropin receptor, FSH R, FSHR, FSHRO, LGR1, ODG1

---

Validation Data Gallery

• 

  WB analysis using 22665-1-AP

  mouse ovary tissue were subjected to SDS PAGE followed by western blot with 22665-1-AP (FSHR antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of DU 145 using 22665-1-AP

  DU 145 cells were subjected to SDS PAGE followed by western blot with 22665-1-AP (FSHR antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of PC-3 using 22665-1-AP

  PC-3 cells were subjected to SDS PAGE followed by western blot with 22665-1-AP (FSHR antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 22665-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 22665-1-AP (FSHR antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of rat ovary using 22665-1-AP

  rat ovary tissue were subjected to SDS PAGE followed by western blot with 22665-1-AP (FSHR antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  IHC staining of mouse ovary using 22665-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse ovary tissue slide using 22665-1-AP (FSHR antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse ovary using 22665-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse ovary tissue slide using 22665-1-AP (FSHR antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of HeLa using 22665-1-AP

  Immunofluorescent analysis of (-20℃ Ethanol) fixed HeLa cells using 22665-1-AP (FSHR antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

• 

  IF Staining of HepG2 using 22665-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using FSHR antibody (22665-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L), CL594-phalloidin (red).

---

Tested Applications

• Positive WB detected in: mouse ovary tissue, rat ovary tissue, DU 145 cells, PC-3 cells, HeLa cells
• Positive IHC detected in: mouse ovary tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: HeLa cells, HepG2 cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:3000
• Immunohistochemistry (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 3 publications below
• WB: See 46 publications below
• IHC: See 15 publications below
• IF: See 49 publications below
• FC: See 3 publications below

Product Information

22665-1-AP targets FSHR in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse, rat, pig, chicken, bovine, sheep, goat, geese
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: FSHR fusion protein Ag18158
• Full Name: follicle stimulating hormone receptor
• Calculated Molecular Weight: 695 aa, 78 kDa
• Observed Molecular Weight: 71-78 kDa
• GenBank Accession Number: BC118548
• Gene Symbol: FSHR
• Gene ID (NCBI): 2492
• RRID: AB_2631204
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

Species	Application	Title
mouse	IF	Cell Res; Blocking FSH inhibits hepatic cholesterol biosynthesis and reduces serum cholesterol.; Authors - Yanjing Guo
rat	WB	J Hazard Mater; GRP78/IRE1 and cGAS/STING pathway crosstalk through CHOP facilitates iodoacetic acid-mediated testosterone decline; Authors - Li Mou
human,mouse	WB	Autophagy; Autophagy regulates differentiation of ovarian granulosa cells through degradation of WT1; Authors - Tong Shao
mouse	IHC	Redox Biol; Novel ovarian endometriosis model causes infertility via iron-mediated oxidative stress in mice.; Authors - Shotaro Hayashi
human	IF	ACS Appl Mater Interfaces; A Novel Three-Dimensional Follicle Culture System Decreases Oxidative Stress and Promotes the Prolonged Culture of Human Granulosa Cells; Authors - Xinyang Zhao
human,mouse	WB,IHC	Free Radic Biol Med; Iron overload modulates follicular microenvironment via ROS/HIF-1α/FSHR signaling; Authors - Yaoqiu Wu

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

---

FH

Nolan (Verified Customer) (04-11-2022)

Antibody works better if you stain at room temperature for 2 hours instead of 4C overnight.

• Applications: Western Blot
• Primary Antibody Dilution: 1:500
• Cell Tissue Type: Mouse FSHR

Related Products

LHCGR Polyclonal antibody

19968-1-AP

androgen receptor Polyclonal antibody

22576-1-AP

CYP19A1 Polyclonal antibody

16554-1-AP

APPL1 Polyclonal antibody

12639-1-AP