FSD2 Polyclonal antibody

FSD2 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Reviews (1)

Cat no : 25609-1-AP

Synonyms

FSD2, RP11 127F21, SPRYD1

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Validation Data Gallery

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  WB analysis of mouse heart using 25609-1-AP

  mouse heart tissue were subjected to SDS PAGE followed by western blot with 25609-1-AP (FSD2 Antibody) at dilution of 1:600 incubated at 4 degree celsius over night.

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  WB analysis using 25609-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 25609-1-AP (FSD2 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of rat heart using 25609-1-AP

  rat heart tissue were subjected to SDS PAGE followed by western blot with 25609-1-AP (FSD2 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  IHC staining of mouse heart using 25609-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 25609-1-AP (FSD2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with .

• 

  IHC staining of mouse heart using 25609-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 25609-1-AP (FSD2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with .

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Tested Applications

• Positive WB detected in: mouse heart tissue, rat heart tissue
• Positive IHC detected in: mouse heart tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:4000
• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

25609-1-AP targets FSD2 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: FSD2 fusion protein Ag22229
• Full Name: fibronectin type III and SPRY domain containing 2
• Calculated Molecular Weight: 749 aa, 85 kDa
• Observed Molecular Weight: 95-105 kDa
• GenBank Accession Number: BC130569
• Gene Symbol: FSD2
• Gene ID (NCBI): 123722
• RRID: AB_2880159
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Zee (Verified Customer) (01-28-2020)

It worked very well with western blot.

• Applications: Western Blot,
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: Mouse heart tissue