FRZB Monoclonal antibody, PBS Only

FRZB Monoclonal Antibody for WB, Indirect ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human, mouse

Applications

WB, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

2B4A1

Cat no : 67081-1-PBS

Synonyms

FIZ, FRE, Frezzled, FRITZ, frizzled related protein, Frizzled related protein 1, FRP, FRP 3, FRZB, FRZB 1, FRZB PEN, FRZB1, FZRB, hFIZ, sFRP 3, SFRP3, SRFP3

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Validation Data Gallery

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  WB analysis using 67081-1-Ig (same clone as 67081-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 67081-1-Ig (FRZB antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67081-1-PBS in a different storage buffer formulation.

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  WB analysis of 4T1 using 67081-1-Ig (same clone as 67081-1-PBS)

  4T1 cells were subjected to SDS PAGE followed by western blot with 67081-1-Ig (FRZB antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67081-1-PBS in a different storage buffer formulation.

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Recommended dilution

Application	Dilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

67081-1-PBS targets FRZB in WB, Indirect ELISA applications and shows reactivity with Human, mouse samples.

• Tested Reactivity: Human, mouse
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen: FRZB fusion protein Ag28147
• Full Name: frizzled-related protein
• Calculated Molecular Weight: 325 aa, 36 kDa
• Observed Molecular Weight: 36 kDa
• GenBank Accession Number: BC027855
• Gene Symbol: FRZB
• Gene ID (NCBI): 2487
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein G purification
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.

Background Information

Frzb (also known as sFRP3) is a member of the secreted frizzled related protein (SFRP) family, originally identified as a chondrogenic factor during bone morphogenesis (PMID: 29209231). It was subsequently shown to be a regulator in Wnt signaling, and also have a role in regulating cell growth and differentiation in specific cell types. FRZB was seen to be acting as an oncogene in some cancer, such as metastatic renal cancer.