FRS3 Polyclonal antibody

FRS3 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

Cat no : 12249-1-AP

Synonyms

SNT 2, FRS2beta, FRS2B, FGFR-signaling adaptor SNT2, FGFR substrate 3

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Validation Data Gallery

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  WB analysis of human placenta using 12249-1-AP

  human placenta tissue were subjected to SDS PAGE followed by western blot with 12249-1-AP (FRS3 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  IHC staining of human skin using 12249-1-AP

  Immunohistochemical analysis of paraffin-embedded human skin tissue slide using 12249-1-AP (FRS3 antibody) at dilution of 1:200 (under 10x lens).

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  IHC staining of human skin using 12249-1-AP

  Immunohistochemical analysis of paraffin-embedded human skin tissue slide using 12249-1-AP (FRS3 antibody) at dilution of 1:200 (under 40x lens).

• 

  IF Staining of PC-3 using 12249-1-AP

  Immunofluorescent analysis of PC-3 cells using 12249-1-AP (FRS3 antibody) at dilution of 1:25 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

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Tested Applications

• Positive WB detected in: human placenta tissue
• Positive IHC detected in: human skin tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: PC-3 cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Immunohistochemistry (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:10-1:100

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

12249-1-AP targets FRS3 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: FRS3 fusion protein Ag2896
• Full Name: fibroblast growth factor receptor substrate 3
• Calculated Molecular Weight: 492 aa, 54 kDa
• Observed Molecular Weight: 54 kDa
• GenBank Accession Number: BC010611
• Gene Symbol: FRS3
• Gene ID (NCBI): 10817
• RRID: AB_2106244
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.