FRS2 Monoclonal antibody, PBS Only

FRS2 Monoclonal Antibody for WB, IF, IHC, Indirect ELISA

Host / Isotype

Mouse / IgG3

Reactivity

human

Applications

WB, IF, IHC, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

4E10H12

Cat no : 66263-1-PBS

Synonyms

FGFR signaling adaptor SNT, FGFR substrate 2, FRS2, FRS2A, FRS2alpha, SNT, SNT 1, SNT1

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Validation Data Gallery

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  WB analysis of MCF-7 using 66263-1-Ig (same clone as 66263-1-PBS)

  MCF-7 cells were subjected to SDS PAGE followed by western blot with 66263-1-Ig (FRS2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66263-1-PBS in a different storage buffer formulation.

• 

  WB analysis of HEK-293 using 66263-1-Ig (same clone as 66263-1-PBS)

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 66263-1-Ig (FRS2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66263-1-PBS in a different storage buffer formulation.

• 

  WB analysis of HeLa using 66263-1-Ig (same clone as 66263-1-PBS)

  HeLa cells were subjected to SDS PAGE followed by western blot with (FRS2 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66263-1-PBS in a different storage buffer formulation.

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  IHC staining of human prostate cancer using 66263-1-Ig (same clone as 66263-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 66263-1-Ig (FRS2 Antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66263-1-PBS in a different storage buffer formulation.

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  IHC staining of human prostate cancer using 66263-1-Ig (same clone as 66263-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 66263-1-Ig (FRS2 Antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66263-1-PBS in a different storage buffer formulation.

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  IF Staining of MCF-7 using 66263-1-Ig (same clone as 66263-1-PBS)

  Immunofluorescent analysis of (-20°C Ethanol) fixed MCF-7 cells using FRS2 antibody (66263-1-Ig, Clone: 4E10H12 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66263-1-PBS in a different storage buffer formulation.

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Recommended dilution

Application	Dilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

66263-1-PBS targets FRS2 in WB, IF, IHC, Indirect ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Mouse / IgG3
• Class: Monoclonal
• Type: Antibody
• Immunogen: FRS2 fusion protein Ag19299
• Full Name: fibroblast growth factor receptor substrate 2
• Calculated Molecular Weight: 60 kDa
• Observed Molecular Weight: 68 kDa
• GenBank Accession Number: BC021562
• Gene Symbol: FRS2
• Gene ID (NCBI): 10818
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.

Background Information

Fibroblast growth factor (FGF) receptor substrate 2 (FRS2) has an alternative name as SNT-1, it is an adapter protein that links activated FGR and NGF receptors to downstream signaling pathways. FGF receptor substrates (FRS2 and FRS3) are key adaptor proteins that mediate FGF-FGFR signalling in benign as well as malignant tissue. FRS2 is a 508 amino-acid protein,which is phosphorylated on tyrosine residues. The molecular weight of non-phosphorylated FRS2 is 57-68 kDa, but phosphorylated FRS2 is 80-90 kDa. Phosphorylation of FRS2 is associated with activation of a number of MAP kinases. Allele-specific regulation of FGFR2 mRNA expression with a mildly increased breast cancer risk has been reported.