FRMPD3 Polyclonal antibody

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  IHC staining of mouse brain using 31635-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 31635-1-AP (FRMPD3 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse brain using 31635-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 31635-1-AP (FRMPD3 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse cerebellum using 31635-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue slide using 31635-1-AP (FRMPD3 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse cerebellum using 31635-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue slide using 31635-1-AP (FRMPD3 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

FRMPD3 Polyclonal Antibody for IHC, ELISA

Cat No. 31635-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

IHC, ELISA

Synonyms

KIAA1817

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Tested Applications

• Positive IHC detected in: mouse brain tissue, mouse cerebellum tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

31635-1-AP targets FRMPD3 in IHC, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: FRMPD3 fusion protein Ag36254
• Full Name: FERM and PDZ domain containing 3
• Calculated Molecular Weight: 199kDa
• Observed Molecular Weight: 199 kDa
• GenBank Accession Number: NM_032428
• Gene Symbol: FRMPD3
• Gene ID (NCBI): 84443
• RRID: AB_3670058
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity Purification
• UNIPROT ID: Q5JV73
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

FRMPD3 encodes a protein that contains a PDZ (post synaptic density protein (PSD95), Drosophila disc large tumor suppressor (Dlg1), and zonula occludens-1 protein (zo-1) domain at the N-terminus followed by a FERM domain. The PDZ domain is thought to function in protein-protein interactions, mainly by binding to specific C-terminal peptides of other proteins. The FERM domain is found in proteins that are localized to the plasma membrane and are associated with the cytoskeleton