FRK Polyclonal antibody

FRK Polyclonal Antibody for WB, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IF/ICC, ELISA

Conjugate

Unconjugated

Publications

1

Cat no : 16197-1-AP

Synonyms

Protein-tyrosine kinase 5, Protein tyrosine kinase 5, GTK, FYN-related kinase, fyn related kinase

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Validation Data Gallery

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  WB analysis using 16197-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 16197-1-AP (FRK antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IF Staining of HepG2 using 16197-1-AP

  Immunofluorescent analysis of HepG2 cells, using FRK antibody 16197-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

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Tested Applications

• Positive WB detected in: mouse kidney tissue, rat kidney tissue
• Positive IF/ICC detected in: HepG2 cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:10-1:100

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 1 publications below

Product Information

16197-1-AP targets FRK in WB, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: FRK fusion protein Ag10372
• Full Name: fyn-related kinase
• Calculated Molecular Weight: 505 aa, 56 kDa
• Observed Molecular Weight: 54-57 kDa
• GenBank Accession Number: BC012916
• Gene Symbol: FRK
• Gene ID (NCBI): 2444
• RRID: AB_10639525
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

The fyn-related kinase (FRK) belongs to the tyrosine kinase family of protein kinases and is also named PTK5 (protein-tyrosine kinase 5), RAK(Nuclear tyrosine protein kinase). The Frk is a tyrosine kinase expressed in various tissues, such as the gastrointestinal tract and pancreatic islet cells. It affects pancreatic beta cell number during embryogenesis and promotes beta cell cytotoxic signals in response to streptozotocin(PMID:20157399). It may function as a tumor suppressor(PMID:19345329).

Publications

Species	Application	Title
human,mouse	WB	Cancer Lett; FLT3-Selective PROTAC: Enhanced Safety and Increased Synergy with Venetoclax in FLT3-ITD Mutated Acute Myeloid Leukemia; Authors - Yuxin Tan