NCS1 Polyclonal antibody

• Featured Product
• KD/KO Validated

NCS1 Polyclonal Antibody for WB, IHC, IF-P, FC (Intra), IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF-P, FC (Intra), IP, CoIP, ELISA

Conjugate

Unconjugated

Publications

3

Cat no : 10506-2-AP

Synonyms

FREQ, FLUP, Frequenin-like protein, Frequenin like protein, frequenin homolog (Drosophila)

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Validation Data Gallery

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  WB analysis of mouse brain using 10506-2-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 10506-2-AP (NCS1 antibody at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of rat brain using 10506-2-AP

  rat brain tissue were subjected to SDS PAGE followed by western blot with 10506-2-AP (NCS1 antibody at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse cerebellum using 10506-2-AP

  mouse cerebellum tissue were subjected to SDS PAGE followed by western blot with 10506-2-AP (NCS1 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HepG2 using 10506-2-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 10506-2-AP (NCS1 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  WB analysis of PC-3 using 10506-2-AP

  PC-3 cells were subjected to SDS PAGE followed by western blot with 10506-2-AP (NCS1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of SH-SY5Y using 10506-2-AP

  SH-SY5Y cells were subjected to SDS PAGE followed by western blot with 10506-2-AP (NCS1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human brain using 10506-2-AP

  human brain tissue were subjected to SDS PAGE followed by western blot with 10506-2-AP (NCS1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of A431 using 10506-2-AP

  A431 cells were subjected to SDS PAGE followed by western blot with 10506-2-AP (NCS1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  IP experiment of mouse brain using 10506-2-AP

  IP result of anti-NCS1 (IP:10506-2-AP, 4ug; Detection:10506-2-AP 1:1000) with mouse brain tissue lysate 4000ug.

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  IHC staining of human cerebellum using 10506-2-AP

  Immunohistochemical analysis of paraffin-embedded human cerebellum tissue slide using 10506-2-AP (NCS1 antibody at dilution of 1:200 (under 10x lens).

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  IHC staining of human cerebellum using 10506-2-AP

  Immunohistochemical analysis of paraffin-embedded human cerebellum tissue slide using 10506-2-AP (NCS1 antibody at dilution of 1:200 (under 40x lens).

• 

  IHC staining of human brain using 10506-2-AP

  Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 10506-2-AP (NCS1 antibody at dilution of 1:200 (under 10x lens).

• 

  IHC staining of human brain using 10506-2-AP

  Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 10506-2-AP (NCS1 antibody at dilution of 1:200 (under 40x lens).

• 

  IF Staining of mouse cerebellum using 10506-2-AP

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse cerebellum tissue using NCS1 antibody (10506-2-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of mouse cerebellum using 10506-2-AP

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse cerebellum tissue using NCS1 antibody (10506-2-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of mouse cerebellum using 10506-2-AP

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse cerebellum tissue using NCS1 antibody (10506-2-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  FC experiment of SH-SY5Y using 10506-2-AP

  1X10^6 SH-SY5Y cells were intracellularly stained with 0.2 ug Anti-Human NCS1 (10506-2-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.2 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Tested Applications

• Positive WB detected in: mouse brain tissue, A431 cells, HepG2 cells, human brain tissue, mouse cerebellum tissue, PC-3 cells, rat brain tissue, SH-SY5Y cells
• Positive IP detected in: mouse brain tissue
• Positive IHC detected in: human cerebellum tissue, human brain tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF-P detected in: mouse cerebellum tissue
• Positive FC (Intra) detected in: SH-SY5Y cells

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:4000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)-P: IF-P : 1:50-1:500
• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.20 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 1 publications below
• WB: See 3 publications below
• CoIP: See 1 publications below

Product Information

10506-2-AP targets NCS1 in WB, IHC, IF-P, FC (Intra), IP, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: NCS1 fusion protein Ag0784
• Full Name: frequenin homolog (Drosophila)
• Calculated Molecular Weight: 22 kDa
• Observed Molecular Weight: 22 kDa
• GenBank Accession Number: BC004856
• Gene Symbol: NCS1
• Gene ID (NCBI): 23413
• RRID: AB_2105918
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

NCS1 is a member of the neuronal calcium sensor gene family. It regulates G protein-coupled receptor phosphorylation in a calcium-dependent manner and can substitute for calmodulin. The protein is associated with secretory granules and modulates synaptic transmission and synaptic plasticity.

Publications

Species	Application	Title
mouse	WB	Neuron; NCS-1 in the dentate gyrus promotes exploration, synaptic plasticity, and rapid acquisition of spatial memory.; Authors - Bechara J Saab
mouse	WB	Sci Rep; Self-directed exploration provides a Ncs1-dependent learning bonus.; Authors - Ho-Suk Mun ; KD Validated
mouse	WB,CoIP	Sci Transl Med; Activation of the sigma-1 receptor chaperone alleviates symptoms of Wolfram syndrome in preclinical models; Authors - Lucie Crouzier

Related Products

Flow Cytometry Perm Buffer (10X)

PF00011-C