FOXO1 Monoclonal antibody, PBS Only

• Featured Product
• KD/KO Validated

FOXO1 Monoclonal Antibody for WB, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human

Applications

WB, ELISA

Conjugate

Unconjugated

CloneNo.

1D9A2

Cat no : 66457-1-PBS

Synonyms

FKH1, FKHR, forkhead box O1, Forkhead box protein O1, Forkhead box protein O1A, Forkhead in rhabdomyosarcoma, FOXO1, FOXO1A

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Validation Data Gallery

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  WB analysis using 66457-1-Ig (same clone as 66457-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 66457-1-Ig (FOXO1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66457-1-PBS in a different storage buffer formulation.

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  WB analysis of HEK-293 using 66457-1-Ig (same clone as 66457-1-PBS)

  WB result of FOXO1 antibody (66457-1-Ig; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-FOXO1 transfected HEK-293 cells. This data was developed using the same antibody clone with 66457-1-PBS in a different storage buffer formulation.

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  WB analysis of HeLa using 66457-1-Ig (same clone as 66457-1-PBS)

  HeLa cells were subjected to SDS PAGE followed by western blot with 66457-1-Ig (FOXO1 antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66457-1-PBS in a different storage buffer formulation.

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  WB analysis of SMMC-7721 using 66457-1-Ig (same clone as 66457-1-PBS)

  SMMC-7721 cells were subjected to SDS PAGE followed by western blot with 66457-1-Ig (FOXO1 antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66457-1-PBS in a different storage buffer formulation.

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Recommended dilution

Application	Dilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

66457-1-PBS targets FOXO1 in WB, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen: FOXO1 fusion protein Ag13296
• Full Name: forkhead box O1
• Calculated Molecular Weight: 655 aa, 70 kDa
• Observed Molecular Weight: 70 kDa
• GenBank Accession Number: BC021981
• Gene Symbol: FOXO1
• Gene ID (NCBI): 2308
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS only
• Storage Conditions: Store at -80°C.

Background Information

FOXO1, also named as FOXO1A, FKHR and FKH1, is a member of the FOXO subfamily of Forkhead transcription factors. FOXO1 is a transcription factor which acts as a regulator of cell responses to oxidative stress. FOXO1 interacts with LRPPRC and SIRT1. In the presence of KIRT1, FOXO1 mediates down-regulation of cyclin D1 and up-regulation of CDKN1B levels which are required for cell transition from proliferative growth to quiescence. FOXO1 contains three predicted protein kinase B phosphorylation sites (Thr-24, Ser-256, and Ser-319) that are conserved in other FOXO proteins. The t(2;13) and the variant t(1;13) translocations generate PAX3/FKHR and PAX7/FKHR fusion proteins respectively. The resulting protein is a transcriptional activator. Defects in FOXO1 are a cause of rhabdomyosarcoma type 2 (RMS2).