FOXN3 Polyclonal antibody

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  WB analysis using 25399-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 25399-1-AP (FOXN3 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  IF Staining of U2OS using 25399-1-AP

  Immunofluorescent analysis of (4% PFA) fixed U2OS cells using FOXN3 antibody (25399-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

FOXN3 Polyclonal Antibody for WB, IF/ICC, ELISA

Cat No. 25399-1-AP

Publications(1)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IF/ICC, ELISA

Synonyms

C14orf116, Checkpoint suppressor 1, CHES1, forkhead box N3, Forkhead box protein N3, FOXN3, PRO1635

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Tested Applications

• Positive WB detected in: HEK-293 cells, HepG2 cells, mouse embryo tissue
• Positive IF/ICC detected in: U2OS cells

Recommended dilution

• Western Blot (WB): WB : 1:200-1:1000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 1 publications below
• WB: See 1 publications below

Product Information

25399-1-AP targets FOXN3 in WB, IF/ICC, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: FOXN3 fusion protein Ag21929
• Full Name: forkhead box N3
• Calculated Molecular Weight: 490 aa, 54 kDa
• Observed Molecular Weight: 54 kDa
• GenBank Accession Number: BC007506
• Gene Symbol: FOXN3
• Gene ID (NCBI): 1112
• RRID: AB_3085795
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: O00409
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

As transcriptional regulators, the FOX protein family regulates the development of tissues, embryos as well as carbohydrate and lipid metabolism. Forkhead box N3 (FOXN3), also known as checkpoint suppressor 1 (CHES1), is initially identified from yeast and belongs to the FOX protein family as it contains a conserved FOX DNA binding domain in its N-terminal(PMID: 36450706). FOXN3 had been dysregulated in many types of malignancies including melanoma, osteosarcoma, hepatocellular carcinoma and breast cancer, and its expression level is strongly associated with progression and prognosis of patients(PMID: 31214487).

Publications

Species	Application	Title
human,mouse	WB	Nat Commun; Phosphorylation of FOXN3 by NEK6 promotes pulmonary fibrosis through Smad signaling; Authors - Jinjin Yu ; KO Validated